At CD BioGlyco, we pay attention to every detail in Lipidomics Analysis Services and strive for perfection in Sphingolipid Analysis Services, evidenced by our many years of successful experience. We provide advanced liquid chromatography with tandem mass spectrometry technology for tetrasialoganglioside 1 (GQ1) analysis.

Structure and Functions of GQ1

GQ1 belongs to the group of gangliosides within sphingolipids. Its structure consists of a ceramide backbone linked to an oligosaccharide unit consisting of eight sugar molecules. Four of these are sialic acids. GQ1 belongs to the group of major gangliosides in the mammalian brain. GQ1 levels are reduced in Alzheimer's disease models, and GQ1 has been shown to interact with cholera toxin.

Fig.1 Fully automated (−) nanoESI chip QTOF MS screening of the native ganglioside mixture extracted from NB tumor tissue. (Robu, et al., 2016)

GQ1 Analysis Service at CD BioGlyco

CD BioGlyco provides advanced LC-MS/MS technology for the analysis of bovine and mouse GQ1 using a phenyl-hexyl HPLC column and a linear methanol gradient in water. Our analysis steps are as follows.

• Preparation of mouse brain sample

A volume of homogenized tissue samples is subjected to a liquid-liquid extraction procedure for ganglioside analysis and an additional solid phase extraction (SPE) procedure. After completing the liquid-liquid extraction step, the collected supernatant is applied to a C18 SPE column.

• Preparation of ganglioside standard solutions

A standard ganglioside solution is run as a calibrator to quantify gangliosides in mouse superior colliculus samples. Prepare a stock solution of gangliosides from bovine brain gangliosides, dissolved in 50% methanol in water. The stock solutions are serially diluted with 50% methanol in water to prepare standard solutions with different concentration gradients.

• HPLC and MS analysis

HPLC analysis is performed using a chromatograph equipped with a phenyl hexyl column and a pre-column. Elution and separation are carried out by optimal analytical procedures.

• Precision and accuracy

The day-to-day precision of ganglioside/internal standard peak area ratios in three standard solutions of gangliosides with low, medium, and high concentrations is determined. Accuracy is assessed by quantitative matrix effects determined by dilution studies.

Advantages of Us

• The phenyl-hexyl HPLC technique adds to the arsenal of existing reversed-phase and HILIC chromatographic techniques, providing a complementary separation capability needed for the analysis of gangliosides in biological samples.
• The present technique has the advantage over both of the currently-used HPLC techniques, combining the hydrophilic separating capability of the HILIC technique with the non-polar separating capability of the reversed-phase technique.
• This technique separates gangliosides based on the amount of sialic acid, and the retention time reflects the amount of sialic acid in the ganglioside.
• This technology has broad application prospects and simultaneously separates GM, GD, GT, and GQ.

CD BioGlyco aims to provide clients with unparalleled support services for Ganglioside Analysis. We provide advanced LC-MS/MS technology for GQ1 analysis. If you are interested in our services, please contact us for more details without any hesitation.

Reference

1. Robu, A.C.; et al. Mass spectrometry of gangliosides in extracranial tumors: Application to adrenal neuroblastoma. Analytical Biochemistry. 2016, 509: 1-11.