CD BioGlyco is a professional Lectin Manufacturer, we have different Trichosanthes kirilowii lectin (TKA) extraction and purification methods, which are suitable for the client's scientific research needs or industrial needs. We provide the production services as follows.

• Extraction and purification services of TKA

We use the buffer salt extraction method to optimize the extraction process continuously and adjust the solution ratio to the salt concentration. Optimize the extraction time to provide clients with the highest quality lectin extraction process in the shortest time. It should be noted that our extraction steps are all conducted under 4°C. Moreover, we provide a combination of DEAE-agarose gel column and chromatography to purify lectin, which is a prerequisite for elucidating their chemical properties and biological activities.

Fig.1 Flowchart of extraction of TKA. (CD BioGlyco)

• Purity and molecular weight determination

At CD BioGlyco, we use a microporous membrane filter to filter the lyophilized powder solution and purified TKA based on the above method. Then, the purity of TKA is analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and stained with coomassie brilliant blue (CBB). Moreover, our trained staff provides molecular weight analysis by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.

• TKA activity

According to the client's different cell therapy programs and unique training conditions, we have a mature training environment and first-class technical personnel. We provide comprehensive and efficient comprehensive services to ensure your customized training will not go wrong. After the drop addition of various concentrations of TKA, the cell groups are analyzed by flow cytometry. In addition, we also provide cell cycle analysis, immunofluorescence analysis, chromatin immunoprecipitation (ChIP) assay, dual-luciferase reporter assay, quantitative real-time PCR assay, western blot, and other analytical detection services.

Fig.2 Schematic diagram of TKA analysis and testing service. (CD BioGlyco)

Applications of TKA

• TKA exerts a hypoglycemic effect in alloxan-induced diabetic mice.
• TKA causes the inhibition of the Notch signaling pathway and increases the proportion of M2 macrophages, thereby alleviating the deterioration of kidney structure and function.
• TKA significantly increased the viability and inhibited apoptosis in high glucose-treated HK-2 cells. In vivo experiments demonstrated that TKA attenuated the histopathological damage and inflammatory response in rat kidney tissue induced by Streptozotocin.

CD BioGlyco is a leading global lectin manufacturing company, that offers clients a full range of Natural Lectin Production Services. Specifically, we provide Plant Lectin Production Service, Animal Lectin Production Service, and Fungal Lectin Production Service. Our manufacturing equipment meets any of your manufacturing requirements from laboratory scale to industrial scale. To learn more about the manufacturing of lectin, please feel free to contact us.

References:

1. Lu, J.D.; et al. Trichosanthes kirilowii lectin alleviates diabetic nephropathy by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway. Biosci Rep. 2018, 38(5): BSR20180071.
2. Lu, J.D.; et al. Trichosanthes kirilowii lectin ameliorates streptozocin-induced kidney injury via modulation of the balance between M1/M2 phenotype macrophage. Biomed Pharmacother. 2019, 109: 93-102.