Characteristics of the Globo-HCer Antigen

Globo-HCer antigen is a tumor-associated carbohydrate antigen, a glycolipid composed of six sugar molecules. It is also an antigen that is overexpressed in many cancers, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. In particular, it can be highly expressed in epithelial cancers. Therefore, based on its ability to be overexpressed on the surface of malignant tumor cells, the Globo-HCer antigen may be a target for novel cancer therapeutics and can be used to synthesize cancer vaccine candidates and induce the formation of antibodies against cancer cells.

Tumor-Associated Globo-HCer Antigen Production Service at CD BioGlyco

CD BioGlyco has a complete Vaccine Development Services Platform and is focusing on the development of various vaccines, such as Carbohydrate Vaccines. The production of relevant antigens is an important part of the vaccine development process. For the development of tumor-related vaccines, CD BioGlyco provides Globo-HCer antigen production services.

• Chemical synthesis
  • Total synthesis
    This synthetic route mainly consists of two steps based on pre-activated glycosylation and modified iodosulfonylimination/rearrangement.
  • Linear synthesis
    We will synthesize the tumor-associated carbohydrate antigen Globo-HCer by a linear approach with a glycosylphosphomonosaccharide structural unit. The highly selective galactosyl phosphate donor mounts the α-(1→4-cis)-galactoside bond.
  • Automated solid-phase synthesis
    Globo-HCer antigen belongs to carbohydrates and this method is more applicable for the synthesis of carbohydrates.
  • One-pot synthesis
    This method employs sequential coupling of four glycosyl structural units, which is simple to operate and has higher efficiency of sugar assembly.
• Enzyme synthesis
  • First pathway
    Several glycosyltransferases are used and combined with sugar nucleotides to synthesize the Globo-HCer antigen.
  • Second pathway
    Globo-HCer antigen is synthesized using a rapidly assembled preactivation-based one-pot glycosylation method in combination with specific glycosyltransferases.

Because Globo-HCer is expressed on many tumors in the human body, we also offer the development of anti-tumor vaccines using Globo-HCer as an antigen in combination with preservatives, stabilizers, surfactants, diluents, and adjuvants.

Fig.1 Methods of producing Globo-HCer antigen. (CD BioGlyco)

Applications

• Globo-HCer antigen is highly expressed on a wide range of tumor cells, so its production can be used to develop tumor-associated vaccines.
• Globo-HCer antigen is a carbohydrate, and its production studies can be used to develop carbohydrate-type antigens.
• Globo-HCer antigen could be used in the therapeutical research of tumors.

Advantages

• We have a variety of Globo-HCer antigen production routes that help researchers produce relevant types of antigens more efficiently.
• Our research team is responsive and timely in helping our clients solve the challenges in research for antigen production and vaccine development.
• We have specialized researchers to develop research protocols that meet our clients' goals.

CD BioGlyco has extensive experience in Globo-HCer antigen production and vaccine development services. If you are interested in our services, please feel free to contact us for more details without any hesitation.

Reference

1. Su, D.M.; et al. Enzymatic synthesis of tumor-associated carbohydrate antigen Globo-H hexasaccharide. Org. Lett. 2008, 10(5): 1009-1012.