Urtica dioica Lectin Production Service

Urtica dioica lectin (UDA) is an acid- and heat-resistant single-chain protein. It contains two Hevein domains and has a higher propensity for chip oligosaccharides. CD BioGlyco has a set of solutions for Lectin Production Service with the support of a professional team.

UDA Extraction and Purification Service at CD BioGlyco

Collect Urtica dioica rhizomes, remove dust, and wash. Then cut into pieces. In a buffer of hydrochloric acid, the rhizomes are homogenized. The homogenized slurry is subjected to filtration and centrifugation, to collect the supernatant. Buffer is added to the supernatant, let at low temperature, and centrifugation-secondary filtration with filter paper to ensure the removal of particulate impurities. The unbound proteins are rinsed in acetate buffer and the lectin is treated with acetate solution as part of chitin column separation. Pick up fractions containing lectin and add (NH4)2SO4. Use centrifugation to collect the precipitates. Dissolve in acetate buffer and apply it to the chitin column. Pick up fractions containing lectin and add (NH4)2SO4. Then apply to the column of s sulfopropyl-sephadex. Unbound proteins are isolated from an acetate buffer, and target products are insulated with a straight line of salt solution.

Fig.1 Flow chart of extraction and purification of UDA. (CD BioGlyco)

Publication

Paper Title: Inhibitory potency of the nettle lectin on neovascularization: a biomolecule for carbohydrate-mediated targeting of angiogenesis

Model: Wound repair model for cell migration assay

Journal: Molecular Biology Reports

Published: 2023

Results: By examining the inhibitory and proliferative effects of different concentrations of UDA on several normal and cancer cell lines, a dose-dependent and increasing inhibition of UDA on HDF and L-929 cells was observed. By examining the expression of MTT, cell migration assay, endothelial cell differentiation, VEGF-A/VEGF-R2, and integrin α 2. The results showed that UDA inhibited the growth but did not interfere with the activity of normal cells. We engineered an in vitro wound repair model showing that UDA had inhibitory migration effects on HUVECs and U87 cell motility. Furthermore, the VEGF-integrin in HUVECs was also associated with UDA treatment, sideways reflecting the antiangiogenic activity of UDA.

Fig.2 Plot of growth inhibition analysis of different cell lines after UDA treatment. (Samadian, et al., 2023)

Applications of UDA

UDA regulates the binding of sex hormone-binding globulin to its receptors on the human prostatic membrane.
UDA has great potential in sugar targeting science, and it is a novel target for immunomodulatory regulation, inhibition of microbial or viral pathogens, and antiproliferative activity against cancer cells.
UDA interferes with cancer cell proliferation and vascularization by binding glycoconjugates containing GlcNAc/Man oligomers.
UDA has activity against benign prostatic hyperplasia (BPH) and therefore has therapeutic value for leukemia.

CD BioGlyco has provided Plant Lectin Production Services for many years. Our clients around the world have praised our products and services. We attach great importance to the extraction process of Natural source products to ensure that the biological activity is not destroyed. Please feel free to contact us if you would like to learn more about the manufacturing of UDA.

Reference:

Samadian, E.; et al. Inhibitory potency of the nettle lectin on neovascularization: a biomolecule for carbohydrate-mediated targeting of angiogenesis. Mol Biol Rep. 2023, 50(5): 4491-4503.

This service is for Research Use Only, not intended for any clinical use.