Vicia villosa Lectin (VVL) Production Services at CD BioGlyco

CD BioGlyco offers a wide variety of Lectin Production Services. Our production services cover the extraction, purification, and full analysis of Plant Lectins, Animal Lectins, and other lectins. Moreover, we have prepared several VVL Products. Among them, the production of VVL is shown below.

• VVL extraction and purification

First, we crush the Vicia villosa seeds. Secondly, the extraction solution is used for extraction. Finally, after centrifugation, the supernatant is collected to obtain the crude VVL.

We use Affinity Chromatography to purify VVL. The monosaccharide is used as the affinity adsorbent, and after attaching the lectin crude to the affinity chromatography column, the lectin is eluted step by step using a salt solution and collecting the eluent, dialyzing, and lyophilizing to obtain the pure product of VVL.

Fig.1 Process of VVL purification. (CD BioGlyco)

• VVL property assay
  • Purity analysis
    VVL is stained and analyzed for purity using polyacrylamide gel electrophoresis.
  • Agglutination activity analysis
    Using the hemagglutination test, VVL is mixed with erythrocyte suspensions from different animals, left to stand, and the coagulation effect of the lectin in each mixture is observed under a microscope.
  • Protein content analysis
    Utilizing a spectrophotometer to detect the absorbance of VVL under the standard vertebrate solution, the protein content is calculated from the absorbance curve.
  • Sugar binding property analysis
    Using different sugar solutions to mix well with VVL, the binding capacity between VVL and sugar is detected according to the radioisotope labeling method.
  • Metal ion binding assay
    Dissolve the lectin with buffer and after dialysis and centrifugation process, add different metal ion solutions to it and assay the hemagglutination activity using hemagglutination assay.
  • Stability assay
    Detection of hemagglutination activity of VVL at different temperatures and pH values using hemagglutination assay to get its stability results.

Fig.2 The main elements included in the VVL production service. (CD BioGlyco)

Applications

• VVL is used to detect and separate glycan conjugates.
• VVL is used to detect physiological processes occurring on the surface of cells, e.g. to distinguish between cells and cancer.
• VVL is used as a model for research on the interaction between glycans and proteins.

Advantages of Us

• We provide lectin production services suitable for research in industries such as drug discovery, biological development, and agricultural science.
• We use state-of-the-art technology and equipment to provide custom production solutions to ensure that our products are of the highest quality.
• Our R&D team is well-versed in lectin purification and analysis and solves complex problems.

CD BioGlyco is in line with the world's advanced technology and provides high-quality lectin production services to clients all over the world. We produce a wide range of lectins, covering Natural Lectins, Recombinant Lectins, and other lectins. Please feel free to contact us if you need high-quality and efficient service.

Reference:

1. Wang, K.; et al. Identification of novel O-linked glycosylated Toxoplasma proteins by Vicia villosa lectin chromatography. PLOS ONE. 2016, 11(3): e0150561.