Vigna radiata Lectin (VRA) Production Services at CD BioGlyco

Lectins are glycoproteins that are capable of agglutinating human and animal red blood cells. Natural lectins are mainly derived from plants, animals, and fungi. For a long time, CD BioGlyco has been providing Plant Lectin, Animal Lectin, and Fungal Lectin production services to clients all over the world based on our advanced technology. Our VRA production services include but are not limited to, the following. We have prepared several Lectin Products and all of them have been tested for quality.

• Separation extraction and purification

Firstly, we crush Vigna radiata and extract VRA by using salt solution as the extracting solution. Secondly, we add a certain amount of phosphate buffer into it to improve the extraction efficiency of the lectin. Finally, the crude extract of VRA is obtained by centrifugation, sedimentation, and filtration.

The crude extract of VRA is double-salted with ammonium sulfate to collect the precipitate. After extraction and dextran gel filtration, high-purity VRA is obtained.

• Property analysis
  • Structural detection
    To detect different levels of structure of VRA using infrared spectroscopy and circular dichroism spectroscopy.
  • Agglutination activity assay
    The agglutination activity of VRA is assayed by coagulation assay. Blood erythrocyte suspensions of different animals are used andVRA is added to them separately. After standing, the coagulant activity is observed.
  • Molecular weight assay
    Sodium dodecyl sulfate-polyacrylamide gel electrophoresis is an effective method for detecting its molecular weight and shows the protein bands clearly.
  • Optimal extraction buffer solution assay
    Different buffers with different concentrations and pH are used for extraction under the same conditions, and the agglutination activity of the extracts is determined separately to determine the optimal extraction buffer solution.
  • Stability test
    The agglutination activity is measured at different temperatures or different pH values to determine the thermal and acid-base stability.
  • Sugar binding assay
    Dissolve VRA in buffer add different types of sugar to the solution separately and assay the hemagglutination activity using the hemagglutination method.

Fig.1 VRA production services. (CD BioGlyco)

Applications

• VRA has glycosyl binding specificity, which allows VRA to be used as research tools such as biological probes to understand life phenomena or pathological changes at the molecular or atomic level.
• VRA is used to probe many important processes such as organogenesis, defense mechanisms, signaling, and stress responses.
• VRA is used in the development of blood clotting agents.

Advantages

• We offer the ability to produceVRA in large quantities. In addition, our supplied products are characterized by high purity and high stability.
• We focus on communication and cooperation with each of our clients and provide them with custom services through an in-depth understanding of their needs.
• Our service team is well versed in various lectin production technologies and offers a high level of service to our clients and solves complex problems.

CD BioGlyco is equipped with advanced Lectin Production technology through continuous innovation. We endeavor to provide the most advanced and high-quality services to each of our clients through a deep understanding of their needs. Please feel free to contact us if you would like to know more about it.

Reference:

1. Coninck, T.D.; Damme, E.J.M.V. Review: The multiple roles of plant lectins. Plant Science. 2021, 313: 111096.