Affinity Chromatography-based Lectin Purification Service at CD BioGlyco

Animal, plant, and other lectins have become increasingly important tools for the study of glycoconjugates in medicine, cell biology, and molecular biology. CD BioGlyco now provides an affinity chromatography-based Lectin Purification Service.

• How does affinity chromatography work?

Affinity chromatography is currently the most commonly used technique for the purification of plant, animal, and other lectins. Most of the currently published affinity purification processes follow the same three steps including binding, washing, and elution. First, a complex solution containing the labeled protein is applied to the column and bound based on the interaction of the affinity matrix with the affinity marker. Next, other non-specifically bound proteins are washed away with a suitable buffer. Finally, the specifically bound proteins are eluted from the affinity column. This process is usually done by cutting off the tag with a protease, competitive binding to similar molecules, or disrupting the interaction of the affinity tag with the matrix.

Fig.1 Schematic diagram of a lectin purification by affinity chromatography. (Silva, 2017)

• Suitable Protocol to Meet Different Experimental Requirements
  • CD BioGlyco will analyze your lectin of interest and select the appropriate affinity chromatography media substrate, coupling method, spacer arm, and ligand to ensure that the ligand reversibly and selectively interacts with the target protein.
  • CD BioGlyco offers a wide variety of spacer arms and ligands to meet different experimental requirements including, but not limited to, peptides, antibodies, proteins, sugars, coenzymes, amino acids, steroids, polynucleotides, and more.
• Alternative Protocol
  • CD BioGlyco uses polymeric macroporous monolithic adsorbents applied in the purification of lectins using affinity chromatography as an alternative to obtaining functional biomolecules with a high degree of purity.
• Other Purification Methods

We have other purification methods available, such as Gel-filtration Chromatography, Hydrophobicity Chromatography, and others. These are customized according to the client's requirements for experimental solutions.

Fig.2 Flow chart of our purification. (CD BioGlyco)

Publication

Applications

• Affinity chromatography technique is detected in western blots, via immunostaining, ELISA assays, or other antibody-based applications.
• The affinity chromatography technique makes use of cells' saccharide components of outer membranes to separate cells into different types.
• Purification of glycoproteins, specifically the membrane-receptor proteins.

Advantages

• Better efficiency resolution.
• Higher pressure stability.
• Better defined column condition.
• Simpler detection & analysis.
• Amenable to smaller samples.
• No loss of protein due to denaturation.
• Suitable for large-scale purification processes.

CD BioGlyco depends on the objective and required purity to optimize methods. Please feel free to contact us if you need chromatographic purification during the production of animal, fungal, and other lectins.