Gel-filtration Chromatography-based Lectin Purification Service at CD BioGlyco

With years of experience in the field of purification, CD BioGlyco now provides a gel-filtration chromatography-based Lectin Purification Service. We customize purification services depending on requestion. Gel chromatography is often used in combination with other chromatography methods.

• How does gel-filtration chromatography work?

Gel filtration chromatography is one of the effective methods for the separation and purification of proteins based on their molecular size. Therefore, it is also known as molecular sieve chromatography. When proteins are purified using gel filtration chromatography, proteins with smaller molecular weights will enter the gel network and their flow will be impeded and slow. However, proteins with larger molecular weights flow out along the spaces between the gel particles. The target proteins are collected at a specific elution time and sequence.

Fig.1 Principle of gel-filtration chromatography. (Du, et al., 2022)

• The steps we take to perform gel filtration chromatography

• Pack gel filtration medium into a column.
• Apply the sample to the column.
• Move the buffer (mobile phase) and sample through the column.
• A buffer is used for elution and the sample is separated between the mobile and stationary phases.
• Smaller molecules enter the gel medium first and are retained longer on the gel column.
• Smaller molecules pass down the column, while larger molecules cannot pass through the gel column.
• Separate and detect the components.

• Characterization

• Measurement of concentration of proteins
  • Lowry method
• SDS-PAGE
  • To identify the fractions' molecular weight
• MALDI-TOF mass spectrometry
  • The determination of the size and molecular weight

• Other Services

• Affinity Chromatography-based Lectin Purification Service
• Ion Exchange Chromatography-based Lectin Purification Service
• Hydrophobicity Chromatography-based Lectin Purification Service

Fig.2 Flow chart of our purification. (CD BioGlyco)

Publication

Applications

• Gel filtration chromatography is usually used in combination with other chromatographic methods in the production of lectins. Researchers have found that combining gel filtration chromatography with affinity chromatography provides new ideas for the development of related drugs by analyzing the three-dimensional structure of antidepressant drugs.
• Gel filtration chromatography has been widely used to separate target peptides or proteins.
• Gel filtration chromatography is the final step in the purification of low molecular weight hyaluronic acid.
• Gel filtration chromatography is the gold standard for the detection of macroprolactin and plays a vital role in medical examinations.
• Gel filtration chromatography is suitable for the separation and purification of proteins with small or large molecular weights.

Advantages

• Gel filtration chromatography offers the advantages of rapid separation, and simplicity of operation, and does not affect the biological activity of the target.
• Gel filtration chromatography is a robust and reliable technique that is ideally suited for the treatment of biomolecules that are sensitive to metal ions, cofactor concentrations, pH changes, and harsh environmental conditions.
• A compelling advantage of gel filtration chromatography is that conditions are altered depending on the type of sample to be purified or the requirements for further purification, storage, or stockpiling. Such changes do not alter the separation.

CD BioGlyco supports our clients in the purification of lectins by utilizing purification techniques developed over many years. We optimize our lectin purification methods according to the goals and purity required by our clients. Please feel free to contact us if gel filtration chromatography purification is required for lectin production.