Cyanogen Bromide (CNBr) Activation-based Cell Surface Chemical Conjugation Glycoengineering Service

CNBr Activation-based Cell Surface Chemical Conjugation Glycoengineering Service at CD BioGlyco

CD BioGlyco has rich experience in Cell Surface Glycoengineering Services. CNBr-activation is widely used in glycoengineering. In order to meet the research needs of our clients in the field of glycobiology, we provide cell surface chemical conjugation glycoengineering service based on CNBr-activation.

Fig.1 Types of our cell surface chemical conjugation glycoengineering service based on CNBr-activation. (CD BioGlyco)

• Affinity matrix preparation service based on CNBr-activation

Affinity chromatography is a chromatographic technique that utilizes the specialized affinity between biomolecules for separation. Researchers create selected affinity matrices by coupling ligands (e.g., antibodies, enzymes, antigens, or receptors). There are a variety of coupling methods. CNBr activation is the most common method for preparing affinity gels, such as cyanogen bromide-activated agarose. We offer CNBr-activation-based preparation of affinity matrices. We immobilize the ligands by the cyanogen bromide method. This coupling reaction can be carried out spontaneously and is widely used.

• Preparation service of polysaccharide and protein coupling systems

The binding of polysaccharides and proteins requires a small number of functional groups: amino groups, imine groups, disulfide bonds, sparing groups, etc. There are various methods available for covalently linking polysaccharides and proteins, such as amine reduction, diazotization chemical reaction, CNBr-activation method, and so on. Among them, CNBr-activation is one of the commonly used methods. We have a complete Glycoengineering Platform. We prepare polysaccharides and protein couplings by CNBr-activation method according to clients' requirements. The biochemical properties, antigenicity, immunogenicity, etc., of this conjugate are also tested.

Publication

Technology: CNBr-activation

Journal: International Journal of Biological Macromolecules

IF: 8.025

Published: 2021

Results: In this study, the biocoupling of high-quality nanoparticles (MNPs) with antibodies was realized using CNBr. This technique is more facile to operate and more cost-effective. The Fe₃O₄ coated by carboxymethyl dextran (CMD) and anti-CD3 monoclonal antibody was coupled by CNBr. Validation results showed that this coupled antibody can be used to isolate high-purity CD3⁺ T lymphocytes from blood.

Fig.2 Isolation of T CD3⁺ cells using anti-CD3-MNP conjugate. (Moradi, et al., 2021)

Applications of CNBr-Activation-based Cell Surface Chemical Conjugation Glycoengineering

• Couplers prepared based on CNBr-activation are highly selective. They are suitable for the isolation and purification of specific components such as proteins, nucleic acids, lectins, etc., from complex biological mixtures.
• CNBr-activation-based polysaccharide and protein coupling system preparation technology play an important role in vaccine development.
• CNBr-activation-based polysaccharide and protein coupling system preparation technology can be used for cell sorting and cell therapy research.

Advantages of Us

• With our rich experience in glycoengineering, we provide efficient CNBr-activation-based cell surface chemical conjugation glycoengineering service.
• The cyanogen bromide products used in our experiments have passed stringent quality tests and meet or exceed industry standards.
• Cyanogen bromide-activated substrates bind ligands under mild conditions. Other derivatives are obtained by further processing the substrate. This enriches the range of applications.

CD BioGlyco has developed a variety of Cell Surface Glycoengineering Services Based on Chemical Conjugation. In addition to CNBr-activation, we offer Click Chemistry-based Cell Surface Chemical Conjugation Glycoengineering and Hydrazone Ligation-based Cell Surface Chemical Conjugation Glycoengineering service. If you are interested in our glycoengineering services, please feel free to contact us for more details about the experimental protocols, products used, and so on.