Principle of "CNBr Method"-based Polysaccharide Conjugation

CNBr is a chemical reagent with the name cyanogen bromide, which is an organic substance containing halogen. CNBr is often used as a reagent for breaking amide bonds in organic synthesis, mainly for the cleavage of hetero-proteins or proteins containing amide bonds to obtain polypeptides or amino acids. In addition, CNBr can also be used in conjugation reactions between polysaccharides and proteins or peptides and is one of the frequently used reagents for the research of polysaccharide-bound proteins. The principle of "CNBr method"-based polysaccharide conjugation is to conjugate polypeptide or amino acid monomers with free amino groups to polysaccharide molecules with amide groups to achieve various conjugation reactions such as polysaccharide-protein and polysaccharide-peptide. This method has the advantages of high selectivity and efficiency and is one of the important methods in the biomedical field.

"CNBr Method"-based Polysaccharide Conjugation Services at CD BioGlyco

As a glycobiology-focused company, CD BioGlyco has a complete Glyco™ Vaccine Development Platform to provide professional One-stop Solutions for Polysaccharide Conjugation service based on multiple Technologies. Our experts are well versed in chemistry-based polysaccharide conjugation, including "CNBr method"-based polysaccharide conjugation, and the following are the specific services we offer:

• "CNBr method"-based polysaccharide conjugation service by the aggregation method
  We utilize the oxygenation reaction of CNBr on some hydroxyl groups on polysaccharides and subsequently prepare covalent bonds by linking compounds with NH₂. Finally, the connected compounds form specific structures and spatial arrangements on the surface, thus changing the biological activity of the molecule.
• "CNBr method"-based polysaccharide conjugation service by a self-assembling method
  We utilize the affinity of polysaccharides and peptides/proteins themselves to conjugate them through non-covalent bonding interactions, making them suitable for conjugation reactions of large-scale molecules.
• "CNBr method"-based polysaccharide conjugation service by a surface modification method
  We achieve surface modification of polysaccharides by introducing new functional groups on the polysaccharide surface through surface modification methods. This is highly specific and flexible for the synthesis of polysaccharide conjugates, and we combine it with other surface modification methods to give more complex structures and properties to the products.

Fig.1 The "CNBr method"-based polysaccharide conjugation. (CD BioGlyco)

Applications

• Enhancing the water solubility of the drug
• Combination with fluorescent markers for fluorescence imaging
• Food packaging and material coating

Advantages of Us

• High specificity and targeted modification
• Professional research team
• Cost-effective services

CD BioGlyco aims to provide high-quality products and personalized service while offering flexible guidance to meet our clients' needs. We focus on providing client-oriented "CNBr method" based polysaccharide conjugation services based on their specific requirements to help them conduct the research efficiently. If you are interested in our services, please contact us for more details.

Reference:

1. Farkaš, P.; et al. Chemical conjugation of biomacromolecules: A mini-review. Chemical Papers. 2010, 64: 683-695.