Our Comprehensive Conjugation Strategy for Glycan Linking to Coat Protein pVIII for Client

CD BioGlyco has developed the LiGA platform to provide clients with more comprehensive glycan interaction analysis services. In the process of LiGA Construction, attaching the glycan to the coat protein pVIII of M13 bacteriophage is a very critical step. Since there is no natural glycosylation for the M13 bacteriophage, the glycan required for the experiment needs to be attached to the bacteriophage by coupling. We use chemical or enzymatic coupling to perform the ligation, thus producing a multivalent Display of the Glycan.

Fig.1 Conjugation strategy for glycan linking to coat protein pVIII. (CD BioGlyco)

• Glycan Chemical Conjugation to Coat Protein pVIII

Glycan is chemically reactive with the reactive functional groups on pVIII. For this reason, we use chemical conjugation to attach the glycan to pVIII, forming a covalent bond between them. Depending on the specific reactive groups, we use different chemical conjugation methods, such as strain-promoted azido alkyne cycloaddition (SPAAC), Cu-activated azido alkyne cycloaddition (CuAAC), and so on.

• Glycan Enzymatic Conjugation to Coat Protein pVIII

Enzymatic conjugation is also an effective method to link glycans and pVIII. Under appropriate reaction conditions, we employ specific enzymes to conjugate pVIII and glycans.

We choose the appropriate conjugation strategy based on the density and structure of the glycan on pVIII. During the conjugation process, we consider the effects of experimental conditions on the stability and functionality of the glycan and pVIII. By optimizing and adjusting the experimental conditions and conjugation method, we finally achieve the ideal density of the glycan. During the experiment, we also adjust the experimental process and introduce modifications to meet the specific research needs of our clients. Finally, we verify the binding affinity and specificity of the conjugate by enzyme-linked immunosorbent assay (ELISA).

Publication Data

Applications of Conjugation Strategy for Glycan Linking to Coat Protein pVIII

• Conjugation of glycans to pVIII allows the display of glycans on bacteriophage surfaces for a variety of applications such as glycan microarrays and protein-glycan interaction studies.
• The LiGA constructed by conjugating glycans to pVIII provides a versatile tool for glycan biology and immunology.
• LiGA constructed by conjugating glycans to pVIII is used for vaccine development.

Advantages of Us

• We screen for appropriate conjugation strategies to ensure the successful coupling of glycans to pVIII as well as to maintain the structural integrity and functionality of both molecules.
• The conjugation of glycans to pVIII is carefully validated and characterized to ensure the reproducibility and consistency of the conjugation process.
• Specific modifications are made to the glycan to meet the needs of a particular application.

CD BioGlyco is dedicated to the research of technologies related to glycan analysis. We optimize each step of the LiGA construction reaction to provide high-quality LiGA analysis services. Please feel free to contact us to learn more about glycan conjugation with coat protein pVIII. Our professional research team will surely give you the most satisfactory answer.