Liquid Glycan Display Array (LiGA)

Expanding Knowledge, Innovating for Better Liquid Glycan Display Array (LiGA)

CD BioGlyco has constructed a complete Glycan Display Platform. LiGA is a technology for high-throughput screening of protein-glycan interactions in vivo and in vitro. This strategy provides the ability to encode and control the density of glycans. With our extensive experience in glycan analysis, we offer LiGA-based analysis services. This process includes glycan modification, LiGA construction, LiGA quality testing, LiGA functional validation, and result analysis.

• LiGA Construction

We construct Engineered M13 Bacteriophages by inserting silent barcodes into the genome of the M13 bacteriophage. Then we Conjugate various glycans into the coat protein pVIII of the M13 bacteriophage. We use enzymatic or chemical coupling methods for the ligation to achieve a multivalent display of the glycans.

• Glycan Preparation and Modification

One of the key steps in the construction of LiGA is the Preparation of Glycan Libraries. We prepare glycan libraries by synthesizing structurally diverse glycans by chemical and enzymatic methods. In addition, glycans isolated from natural sources are also used to prepare glycan libraries.

• Quality Control of LiGA

During the conjugation process, we consider the effect of experimental conditions on the density, stability, functionality of the glycan, etc.

• Functional Validation of LiGA

We use enzyme-linked immunosorbent assay to validate the functional integrity of the glycan on phage.

• LiGA-based analysis service

LiGA utilizes a mixture of multivalent probes of uniform size to assay glycan-protein interactions. Each of these probes has a well-defined glycan density and DNA barcode. We offer the following LiGA-based analysis services.

• LiGA-based Glycan-binding Profiling
• LiGA-based Cell Surface Lectin-binding Profiling
• LiGA-based Glycan Binding Response Profiling

• Analysis of Results

We use fluorescently labeled detection molecules or other assays to detect the binding of proteins to specific glycans. We quantify the signal generated by the bound protein and analyze the affinity and specificity of the protein for the glycan.

Fig.1 Processes for LiGA-based analysis services. (CD BioGlyco)

Publication Data

Applications of LiGA

• LiGA is used to study specific interactions between glycan-binding proteins such as lectins, antibodies, enzymes, and various glycans.
• LiGA is used in studies exploring glycan recognition in various glycan-related biological pathways, immune responses, and cell surface interactions.
• LiGA is used for screening and drug discovery of glycan-based therapeutics. By screening glycan libraries against target proteins or receptors, potential lead compounds for diagnostic or therapeutic interventions are identified.
• Our constructed LiGAs are used in competitive binding assays, kinetic assays, binding assays, and inhibition assays to comprehensively characterize glycan-protein interactions.

Advantages of Us

• We provide one-stop LiGA analysis services from LiGA construction, and glycan binding assay to data analysis.
• Our constructed LiGAs offer multiple advantages, such as the flexibility of glycan libraries, dynamic range, and the ability to analyze complex glycan samples.
• Our constructed LiGA provides a valuable platform for studying glycan binding events, identifying glycan recognition patterns, and elucidating their roles in cellular processes and disease mechanisms.

CD BioGlyco aims to facilitate glycan research by developing multiple glycan display arrays. In addition to LiGA, we also offer Neoglycolipid Display Array-based analysis services. Please feel free to contact us for the detailed process of LiGA construction and so on if you are interested in our LiGA analysis service. Our professional team will do our best to provide the best solution.