Quality Control of LiGA

Promote Your Project Development with LiGA Quality Control

CD BioGlyco is a leading company in the glycobiology industry. We have a high-quality Glycan Display Platform to help clients quickly construct various arrays such as Liquid Glycan Display Array (LiGA). Quality control and Functional Validation of the constructed arrays is very important. This information confirms the conjugation of diverse glycan structures and the biological function of the different structures. Therefore, we provide a comprehensive quality analysis service of LiGA.

Based on our quality control of LiGA, our platform provides the following services

Optimization of wash using colorimetric reporter phage

Our researchers use optimization of wash using colorimetric reporter phage to test four different phage clones simultaneously without sequencing. Meanwhile, we provide testing of the input and output quantities of phages. This service makes the project go more smoothly, and it is very convenient.

Analysis services

Quality control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS): Our investigators perform a detailed analysis of the glycosylation degree of phage samples by MALDI-TOF-MS and provide coupling analysis services for different glycan structures.
Quality control by deep sequencing: Through deep sequencing analysis, we provide clients with molecular binding properties between glycans and glycoproteins. The service report contains key binding information in vitro or in vivo regarding glycoprotein interactions of glycan and cellular reality.
We also provide the below services:

Cellular genomic material analysis service	Agarose gel electrophoresis assay service
Quantification of the chemical modifications on the phage surface	Modification service of the phage cloning
Polymerase chain reaction (PCR) analysis service	Panning service of LiGA in mice
Plaque forming units (PFU) analysis service	Flow cytometry analysis service

Fig.1 Schematic representation of the analytical service tool. (CD BioGlyco)

Publication

Journal: Nature Chemical Biology

Technology: MALDI-TOF-MS, Deep sequencing

Published: 2021

IF: 16.284

Results: In this study, constructed LiGAs were acylated using dibenzazo-cyclooctyl-N-hydroxysuccinimide ester (DBCO-NHS), and their linkage was detected by MALDI-TOF-MS. The results indicate that the degree of coupling interferes with binding to antibodies or glycoproteins, presumably due to the spacing of pVIII on the phage surface. In addition, the coupling of different glycan structures was determined by MALDI analysis (shown below). It also provided information on the position of the amide bond mainly on the N-terminal amine.

Fig.2 Representative spectra of chemical modification of coat protein pVIII with glycans of different structural complexity. (Sojitra, et al., 2021)

Applications

Quality control of LiGA is a useful tool to identify the conjugation of diverse molecular structures.
Quality control of LiGA can be used for in vivo or in vitro surface molecular recognition analysis with living cells.
Quality control of LiGA can be used to decode the best structure and density for identifying glycans.

Advantages of Us

We offer comprehensive LiGA development services, from Construction, Modification, and quality control to functional validation.
Our research team continues to optimize analytical services to ensure the most convenient and useful service to clients.

CD BioGlyco has extensive experience in glycan display. Equipped with high-throughput screening instruments, we are committed to meeting the efficient needs of our clients. We want to be your first choice for array analysis. To learn more information about LiGA, please feel free to contact us.

References

Sojitra, M.; et al. Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage. Nat Chem Biol. 2021, 17(7): 806-816.
Lim, T.S.; Montague-Cardoso, K. Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns. Commun Biol. 2021, 4(1): 976.

This service is for Research Use Only, not intended for any clinical use.