CD BioGlyco is a leading company in the glycobiology industry. We have a high-quality Glycan Display Platform to help clients quickly construct various arrays such as Liquid Glycan Display Array (LiGA). Quality control and Functional Validation of the constructed arrays is very important. This information confirms the conjugation of diverse glycan structures and the biological function of the different structures. Therefore, we provide a comprehensive quality analysis service of LiGA.
Based on our quality control of LiGA, our platform provides the following services
Our researchers use optimization of wash using colorimetric reporter phage to test four different phage clones simultaneously without sequencing. Meanwhile, we provide testing of the input and output quantities of phages. This service makes the project go more smoothly, and it is very convenient.
|
|
|
|
|
|
|
|
Fig.1 Schematic representation of the analytical service tool. (CD BioGlyco)
Journal: Nature Chemical Biology
Technology: MALDI-TOF-MS, Deep sequencing
Published: 2021
IF: 16.284
Results: In this study, constructed LiGAs were acylated using dibenzazo-cyclooctyl-N-hydroxysuccinimide ester (DBCO-NHS), and their linkage was detected by MALDI-TOF-MS. The results indicate that the degree of coupling interferes with binding to antibodies or glycoproteins, presumably due to the spacing of pVIII on the phage surface. In addition, the coupling of different glycan structures was determined by MALDI analysis (shown below). It also provided information on the position of the amide bond mainly on the N-terminal amine.
Fig.2 Representative spectra of chemical modification of coat protein pVIII with glycans of different structural complexity. (Sojitra, et al., 2021)
CD BioGlyco has extensive experience in glycan display. Equipped with high-throughput screening instruments, we are committed to meeting the efficient needs of our clients. We want to be your first choice for array analysis. To learn more information about LiGA, please feel free to contact us.
References