Client Satisfaction with Engineered M13 Bacteriophage Construction Service Is Our Motivation

CD BioGlyco is experienced in Glycan Display for LiGA Construction, LiGA-based research, etc. M13 bacteriophages are used for linkage with glycans in LiGA because of their ease of handling and relatively simple genome. We provide an engineered M13 bacteriophage construction service for LiGA.

To analyze differences in glycan binding patterns, it is necessary to differentiate between M13 bacteriophages carrying glycans. Silent barcodes are a set of DNA fragments with different DNA sequences but encoding chemically identical peptides. We insert silent barcodes into the M13 bacteriophage genome to distinguish between the multiple glycans bound. Silent barcodes can be introduced at sequences excised from bacteriophage proteins or at any conserved position in the bacteriophage vector, including silent regions, translationally active regions, and so on. We primarily place silent barcodes close to variable regions for simultaneous characterization by deep sequencing of short amplicons. M13 bacteriophage libraries with different barcodes are chemically modified and they are combined to form hybrid libraries that are used for glycan analysis.

The construction of an engineered M13 bacteriophage involves the insertion of a silent barcode, chemical modification of the bacteriophage, and functional detection. The engineered M13 bacteriophage constructed through a series of steps is used for LiGA construction.

• Insertion of silent barcode
  We insert silent barcodes into M13 bacteriophage by genetic engineering. DNA constructs containing silent barcodes are constructed using advanced genetic engineering techniques. Afterward, it is transformed into Escherichia coli M13 bacteriophage infected for culture and amplification. Finally, M13 bacteriophage is isolated from E. coli to obtain a large amount of engineered M13 bacteriophage.
• Gene confirmation
  The presence of the desired genetic modifications in the constructed engineered M13 bacteriophage is confirmed by DNA sequencing and other genetic analyses.
• Chemical modification of bacteriophage
  Additional modifications to the glycan-conjugated coat protein pVIII may be required to meet the needs of specific applications. We chemically modify the M13 bacteriophage as requested by the client to meet the needs of the LiGA application.
• Functional validation
  To provide a high-quality engineered M13 bacteriophage construction service, we validate engineered M13 bacteriophage functionality.

Fig.1 Process of constructing engineered bacteriophage M13. (CD BioGlyco)

Publication Data

Applications of Engineered M13 Bacteriophage Construction

• Engineered M13 bacteriophage is successfully constructed and used for bacteriophage display, targeted drug delivery, and so on.
• Engineered M13 bacteriophage is successfully constructed and used for biosensing or bioimaging.
• Engineered M13 bacteriophage is designed to display specific proteins or peptides on its surface to interact with target molecules or cells.

Advantages of Us

• We provide a one-stop engineered M13 bacteriophage construction service.
• The specific protocols and techniques for engineered M13 bacteriophage construction will vary depending on the intended application.
• We are skilled in a variety of advanced genetic engineering tools, such as CRISPR-Cas9 gene editing, to facilitate precise genetic modification of the M13 bacteriophage genome.

CD BioGlyco is committed to expanding the application of glycan display arrays through a variety of advanced tools and experimental methods. Please feel free to contact us to learn more about the construction of engineered M13 bacteriophage, the related genetic engineering techniques used, and other information. We look forward to providing you with this construction service.