In Liquid Glycan Display Array (LiGA), glycans are presented by barcoded M13 virions to allow rapid identification of binding partners based on sequence identity. CD BioGlyco has achieved the purpose of manipulating glycan binding profiles under different conditions by analyzing the linkage between glycans and matching DNA sequences in the phage genome. Moreover, we provide LiGA-based Glycan-binding Profiling and LiGA-based Cell Surface Lectin-binding Profiling.
At CD BioGlyco, our scientists provide systematic analysis services for glycan binding response profiling. Based on LiGA, we highlight the optimal recognition units and optimal densities by profiling the bimodal density-dependent response and affinity-dependent response. Notably, we provide both glycoconjugate and LiGA fractions that are Quality Controlled by matrix-assisted laser desorption/ionization (MALDI) to ensure the accuracy and concordance of the arrays. The services we provide are as follows:
In the same glycan type, our researchers reveal a bimodal density-dependent response by analyzing the binding of LiGA components to different targets (e.g. Concanavalin A, galectin). We provide binding degree testing services for phages at different densities. The relevant spectral data are analyzed by professionals and then sent to the client's email address.
In addition, we offer affinity-dependent response profiling by target binding. We provide analysis and validation of phage and protein binding mechanisms. Our synthetic hybrid phages are used to verify the authenticity of the steric occlusion response mechanism.
Fig.1 Schematic diagram of LiGA-based glycan binding response profiling. (CD BioGlyco)
Technology: Deep sequencing, MALDI spectra, Differential enrichment (DE) analysis
Published: 2021
IF: 16.284
Results: First, researchers synthesized a new LiGA9×6 with 9 glycans (up to a density of 6.) The enrichment of specific glycans was analyzed by deep sequencing. The binding of phages with high-density Manα1-6 (Manα1-3) Manα1- (>300/300) was lower than that of phages containing medium density (130-300) phages. In addition, researchers constructed a hybrid phage containing both producing and non-producing glycans. Analysis shows that the binding to productive glycans decreases when the density of non-productive glycans is increased.
Fig.2 LiGA measures affinity and avidity-based responses in purified GBPs. (Sojitra, et al., 2021)
CD BioGlyco values the client's project characteristics. Our staff customize the personalized service plan according to their different needs. Please feel free to contact us if you are interested in our service.
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