Principle of Crosslinking-based Polysaccharide Conjugation

Polysaccharide conjugation is the binding of a sugar molecule to other biological molecules, like proteins, lipids, or nucleic acids. This binding is usually catalyzed by enzymes that form covalent bond connections. Crosslinking-based polysaccharide conjugation is a chemical reaction that uses crosslinking agents to form crosslinked structures between polysaccharides or within polysaccharide chains. When polysaccharides conjugate with other molecules, they crosslink together by forming covalent bonds, thus affecting the structure and function of the molecule. In crosslinking reactions, cross-linking agents including carboxylic acids, amines, and thiols are frequently used. These crosslinking agents chemically react with polysaccharides to form crosslinking sites that connect polysaccharide chains or polysaccharide molecules. This cross-linked structure enhances the stability of the polysaccharide, modifies its physical and chemical properties and structure, as well as improves biological activity and targeting, and affects other biomolecules interacting with the polysaccharide.

Crosslinking-based Polysaccharide Conjugation Service at CD BioGlyco

CD BioGlyco has established a comprehensive Glyco™ Vaccine Development Platform to provide polysaccharide conjugation services by using advanced Technologies such as crosslinking-based methods. We are able to crosslink a wide range of polysaccharides and prepare high-quality products. The services we provide are as follows:

• Polysaccharide crosslinking conjugation service
  The polysaccharide molecules produce glycoconjugates between them, and these glycoconjugates can be cross-linked between the same polysaccharides or between different polysaccharides. Through crosslinked conjugation, other functional groups or drug molecules can be introduced into the polysaccharide, giving it new functions like bioactivity and cell adhesion properties.
• Intra-chain polysaccharide crosslinking service
  We offer crosslinking conjugation within the same polysaccharide molecule, where the polysaccharide molecule forms a cyclic or highly branched structure. It regulates the solubility, viscosity, degradability, and other properties of polysaccharides, further expanding the range of polysaccharide applications.
• Polysaccharide linear and cross-linking coexistence service
  The products have both linear and crosslinked structures. This coexistence of linear and crosslinked polysaccharides increases the spatial structure and stability of polysaccharides, thus affecting the expression of their biological functions.

Fig.1 Classification of polysaccharide conjugation based on cross-linking. (CD BioGlyco)

Applications

• As an immunomodulator
• As polysaccharide conjugates with biosensing properties
• Thickening agents in foods

Advantages of Us

• Customized synthesis routes of polysaccharide conjugation
• Fewer additives impact the research
• Efficient and reliable service

CD BioGlyco offers a customized crosslinking-based Polysaccharide Conjugation service to meet clients' needs. Moreover, we perform performance testing and analysis of the conjugation products to ensure that the desired functional and application requirements are met. If you have a demand for scientific assistance, please feel free to contact us for further consultation.

Reference:

1. Akhtar, M.; et al. Covalently cross-linked proteins & polysaccharides: Formation, characterisation and potential applications. Current Opinion in Colloid & Interface Science. 2017, 28: 31-36.