DNA is the fundamental storehouse of biological information in living organisms. It plays a key role in the development, growth, and functions of all known life forms. Since it was found, it has been an important investigative tool in many fields including chemical science and life science. For example, there have been significant advancements in synthetic genomics, particularly in the generation of viable microorganisms and other biological systems from synthetic genomes. Besides, a variety of DNA-based nanocarriers have been created that possess the internal features of DNA, including molecular recognition proficiency, structural flexibility, nanoscale controllability, biocompatibility, and biodegradability. These specially designated substances have been combined with many complex structural molecules, such as inorganic nanoparticles, biopolymers, and synthetic polymers to bestow functional properties onto nanocarriers. Therefore, a large number of DNAs are needed. Many technologies are developed to provide DNA in greater purity and quantity.
CD BioGlyco provides diverse methods for the custom synthesis of DNA, including chemical custom DNA synthesis and enzymatic custom DNA synthesis.
In our chemical method, we couple DNA phosphoramidite nucleosides to a solid support to construct an oligonucleotide chain. There is a four-step cycle that includes deblocking, activation, capping, and oxidation. That is used to attach each new nucleoside to the growing oligonucleotide. After formation, the entire polynucleotide is separated from the solid support. Deprotection and other post-synthesis processing are then performed.
Custom Enzymatic DNA Synthesis
Our enzymatic synthesis utilizes terminal deoxynucleotidyl transferase (TdT) as a DNA synthesis tool. The enzymatic method minimizes side product formation and DNA damage by utilizing enzyme specificity and mild conditions. As a result, longer DNA can be directly synthesized without compromising quality.
We also provide synthesis services for some DNA-hybrids that combine DNA with inorganic nanomaterials, including gold nanomaterial, magnetic nanoparticles, and silver nanomaterial. Those hybrids have potential as future material substitutes for some practical bio-applications. Those hybrids have made remarkable advancements in a wide range of cutting-edge applications, particularly in the field of biology. They arouse substantial interest from researchers.
CD BioGlyco is an excellent company in the field of glycobiology, we happily offer tailored solutions to meet clients' specific requirements. Our company excels in diverse carbohydrate synthesis. Our team has leading academic authorities who have made significant contributions to the field. We also have deep knowledge and extensive experience to guarantee the highest level of expertise in custom DNA synthesis. If you are interested in our services, please do not hesitate to contact us.
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