Dive Deep into Dextran with Precision and Clarity
Dextran is a homopolymer of glucose produced by the fermentation of Leuonostoc mesenteroide, containing mainly α-(1,6) and α-(1,4) glycosidic bonds. Depending on the characteristics of the strain-producing dextran, the resulting dextran has different branching units and properties. Therefore, it is necessary to analyze dextran with different molecular weights. CD BioGlyco has a professional research team to provide Pharmaceutical and Biological analysis services for clients.
- Basic test
- CD BioGlyco provides basic testing services such as color, clarity, chloride content, nitrogen content, solubility, and specific rotation determination services.
- CD BioGlyco provides rapid identification services. Mix the configured dextran solution with sodium hydroxide reagent and add a small amount of copper sulfate reagent dropwise to observe the precipitation. Observing the change in precipitation after heating provides a quick indicator of dextran presence.
- Structure and molecular weight analysis
- Our researchers use optical rotation, infrared spectroscopy, and periodate-oxidation reactions for the preliminary structural analysis of dextran. The retention time is characterized in combination with methylation analysis and separation by gas chromatography (GC).
- In particular, we offer capillary gas-liquid chromatography/mass spectrometry (GLC-MS) analysis, thin-layer chromatography (TLC) analysis, high-performance liquid chromatography (HPLC) analysis, nuclear magnetic resonance (NMR) spectra analysis, and two-dimensional (2D) NMR spectroscopy analysis.
- We provide weight average molecular weight testing services by light scattering, ultracentrifugation, small angle neutron scattering, and viscosity. Quantity average molecular weight is accurately provided by membrane osmometry and end-group analysis. In addition, we offer size exclusion chromatography (SEC) to analyze the molecular weight distribution of samples.
- Physico-chemical characteristic analysis
- For low molecular weight dextran that behaves as a random coil in aqueous solution, we offer small angle X-ray scattering (SAXS) measurements, and electron and X-ray diffraction analysis services.
Fig.1 Schematic diagram of dextran analysis service. (CD BioGlyco)
Publication Data
Technology: UV microplate spectrophotometer, Scanning electron microscopy (SEM)
Journal: Public Library of Science ONE
Published: 2016
IF: 3.7
Results: The researchers synthesized and tested several molecular masses of dextran under different conditions. Multiple analyses showed that 70 kDa of dextran was the best for coupling to nanoparticles (NPs). The researchers also modified dextran to act as an NP backbone. Dextran-NPs, which are very promising nanocarriers, have been analyzed and shown to have the advantage of allowing rapid release of the drug.
Fig.2 Mobility-equivalent diameter distribution of NPs. (Wasiak, et al., 2016)
Advantages of Us
- According to the different properties of Pharmaceuticals, we provide comprehensive analytical services including physicochemical analysis, biological activity analysis, etc.
- Customize the testing procedure and accelerate the detection progress according to the client's project and special needs.
- Our professional analytical team provides accurate analysis services and delivers analysis reports to clients for the first time.
CD BioGlyco recommends the appropriate testing content and formulates testing programs for clients. Moreover, we provide high-quality Iron Dextran Analysis service. Please feel free to contact us if you have additional questions about dextran analysis.
References
- Wasiak, I.; et al. Dextran nanoparticle synthesis and properties. PLoS One. 2016, 11(1): e0146237.
- Díaz-Montes, E. Dextran: sources, structures, and properties. Polysaccharides. 2021, 2(3): 554-565.
- Heinze, T.; et al. Functional polymers based on dextran. Polysaccharides Ii. 2006, 199-291.
This service is for Research Use Only, not intended for any clinical use.
