CD BioGlyco provides systematic and one-stop Antibody Digestion Services for the digestion of IgG below the hinge region and ensures the continuity and transparency of the experimental process.
It is very useful to study or exploit the partial activity of immunoglobulins without interference from other molecular fragments. Immunoglobulin molecules are selectively cleaved into fragments with discrete features.
F(ab')2 fragments refer to two antigen-binding F(ab) moieties linked together by disulfide bonds. Compared to whole IgG molecules, F(ab')2 fragments are smaller, allowing better penetration into tissues and facilitating better antigen recognition in IHC. The use of F(ab')2 fragments also avoids non-specific binding to specific receptors (Fc receptors) or specific proteins (Protein A/G) on living cells.
Fig.1 The structure of F(ab')2 fragments. (Bates & Power, 2019)
The fragment crystalline region (Fc region) mainly binds to Fc receptors on the cell surface and interacts with some proteins of the complement system. This property is closely related to the activation of the immune system by antibodies. Therefore, glycosylation of Fc fragments is critical for Fc receptor-mediated activity. In the IgG antibody isotype, the Fc region consists of two identical protein fragments derived from the second and third constant domains of the two heavy chains of the antibody.
CD BioGlyco provides an enzyme that digests IgG at a specific site below the hinge region under physiological reaction conditions, with optimal activity at 37°C. Digestion produces a homogeneous library of F(ab')2 and Fc/2 fragments. As far as we know IgG is the only substrate. In general, IgG is digested within half an hour, but not over-digested with longer incubation times.
Our high specific enzyme can digest all subclasses of human, monkey, rabbit, and sheep IgG, but it has limited activity on mouse IgG2a and IgG3. We will introduce related services on another page: Digestion of Mouse IgG2a and IgG3 Below the Hinge Region.
CD BioGlyco has an advanced Glycoengineering Platform in glycoscience. Our professional research teams allow us to provide you with high-quality services for the digestion of IgG below the hinge region. If you are interested in our services, please contact us for more details without any hesitation.
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