CD BioGlyco provides high-quality Antibody Digestion Services for the digestion of mouse IgG2a and IgG3 below the hinge region research and ensures the continuity and transparency of the experimental process.
Digestion of immunoglobulins has been widely used to prepare bivalent IgG antibody fragments lacking the Fc region. Studies have demonstrated that such F(ab')2 fragments can be used to analyze the role of Fc fragments in various biological functions. Digestive digestion has been applied to immunoglobulins from various species, including rabbits, humans, and mice.
With the development of hybridoma technology, the use of mouse antibodies with specificity and subclasses has greatly increased, and it has become particularly important to understand the effect of pepsin on the various subclasses of mouse IgG. In mice, the IgG class is divided into five subclasses (IgG1, IgG2a, IgG2b, IgG2c, and IgG3). It is worth mentioning that there is no general relationship between the subclasses of each species. However, enzymes that digest mouse IgG2a and IgG3 have yet to be developed.
Fig.1 Mouse IgG subclasses. (Temming, et al., 2020)
CD BioGlyco provides a protease for the preparation of F(ab')2 from mouse subclasses IgG2a and IgG3. The protease is most effective at or near neutral pH and low NaCl concentrations. The protease is fast and easy to use and it digests mouse IgG2a and IgG3 at a specific site below the hinge region to generate intact F(ab')2 and Fc fragments. Since IgG is digested under mild conditions, its immunoreactivity is preserved.
For the digestion of human IgG, we recommend related services on our other webpage: Digestion of IgG Below the Hinge Region.
CD BioGlyco has developed an advanced Glycoengineering Platform in glycoscience. Our professional research teams allow us to provide you with one-stop services for the digestion of mouse IgG2a and IgG3 below the hinge region. If you are interested in our services, please contact us for more details without any hesitation.
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