CD BioGlyco provides disulfide bond formation-based polysaccharide conjugation services for our clients to help them prepare high-quality target molecules with specific structures and functions. We provide clients with relevant professional advice and suggestions to meet their needs in different fields and applications.

Principle of Polysaccharide Conjugation

Polysaccharide conjugation is a biochemical reaction, also known as a glycosyl transfer reaction. In this chemical reaction, a sugar molecule is bound to another biomolecule, such as a protein or nucleic acid, by an acyl ester bond. This reaction is widespread in living organisms and is a very important biochemical process. The formation of disulfide bonds plays a crucial role in polysaccharide affixation reactions. Disulfide bonds are covalent bonds made by combining two sulfur atoms, which strengthen the cross-linking between polysaccharide molecules and make the polysaccharides interact more strongly with each other. In the process of conjugation, when the units containing sulfhydryl groups on two polysaccharide molecules meet, a disulfide bond is formed between them, resulting in a covalent cross-linking between the two polysaccharide molecules, thus producing a polysaccharide conjugation.

Disulfide Bond Formation-based Polysaccharide Conjugation Service at CD BioGlyco

In polysaccharide conjugation reactions, the properties of polysaccharides can be modified, such as enhancing their potency or stability, to meet different drug requirements. At CD BioGlyco, we provide professional One-stop Solutions for Polysaccharide Conjugation services based on many Technologies, including the disulfide bond formation method, and the specific services we offer include the following:

• Using disulfide exchange reactions to form disulfide bonds
  The mild conditions of the disulfide exchange reaction make it suitable for different kinds of polysaccharide conjugates. The extent of the exchange reaction can be controlled by adjusting the reaction conditions to obtain polysaccharide adducts with different structures and properties.
• Using bifunctional crosslinkers to form disulfide bonds
  The products are acid, base, and temperature resistant, with improved chemical and biological stability, and the disulfide bonds formed also exhibit higher stability.
• Using sulfur-containing monofunctional crosslinkers to form disulfide bonds
  Polysaccharides can be effectively bonded together under mild conditions. The cross-linking of sulfur-containing monofunctional crosslinkers does not cause adverse reactions, such as immune rejection of the organism, and has good biocompatibility.

Fig.1 Synthesis process of polysaccharide conjugation. (CD BioGlyco)

Applications

• Preparation of drug slow-release agents
• Increase the nutritional value and functionality of food
• Preparation of new functional materials

Advantages of Us

• Efficient and controllable
• Excellent biocompatibility
• Professional research and service team

CD BioGlyco utilizes advanced technology and a Glyco™ Vaccine Development Platform to provide clients with disulfide bond formation-based polysaccharide conjugation services. Our services offer you effective support and a powerful aid to your research efforts. If you are interested in our services, please feel free to contact us for more details.

Reference:

1. Zhou, Y.; et al. Chemical synthesis of polysaccharide-protein and polysaccharide-peptide conjugates: A review. Carbohydr Polym. 2021, 274: 118662.