What Is DMTMM?

An organic triazine derivative known as 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) is often used to activate carboxylic acids, notably for the manufacture of amides. One of the most common reactions in organic chemistry is amide coupling, and one of the reagents utilized in that process is DMTMM. Additionally, various carboxylic functional groups including esters and anhydrides have been created using DMTMM. And typically, DMTMM is utilized in the chloride form.

Fig.1 DMTMM Chemical Structure. (Wikipedia)

In contrast to the EDC/NHS system, DMTMM is a coupling agent that generates stable activated carboxylic acids with limited alcohol reactivity while remaining water-soluble and resistant to hydrolysis. Hyaluronic acid and alginate were two polysaccharides that underwent amide condensation and modification using DMTMM.

Fig.2 Peptide conjugation to alginate with the help of EDC/NHS coupling. (a) scheme of the reaction; (b) ¹H NMR spectrum of sodium alginate measured in D₂O at 50°C. (Golunova, et al., 2021)

DMTMM-based Polysaccharide Conjugation Services at CD BioGlyco

Nowadays, DMTMM-based polysaccharide conjugation is a hot topic for glycan-relevant research and development. Based on so many advantages of DMTMM shown below (Fig.3), CD BioGlyco develops DMTMM-based polysaccharide conjugation services for our clients. CD BioGlyco has the ability to use DMTMM to modify glycan. On our website, clients will find a One-stop Polysaccharides Conjugation Modification Platform that provides Chemistry-based Polysaccharide Conjugation Services and Physical Crosslinking-based Polysaccharide Conjugation Services.

Fig.3 Advantages of DMTMM-based polysaccharide conjugation. (CD BioGlyco)

Advantages of Us

• Our service team demonstrates excellent problem-solving skills, quickly resolving any issues or challenges that arise and ensuring smooth and seamless service delivery.
• Our lab team demonstrates exceptional technical expertise and a strong work ethic, and we aim to offer excellent DMTMM-based polysaccharide conjugation service for our clients.
• We have extensive research experience in glycan study, and our research and service team provides professional and custom production and analysis services for our clients.

CD BioGlyco provides DMTMM-based polysaccharide conjugation services for clients. This technology has advantages such as high efficiency, mild reaction conditions, high selectivity, good stability of conjugates, versatility, and scalability. These advantages make it a valuable tool for the development of polysaccharide conjugates for various applications in areas such as biomedicine, drug delivery, diagnostics, and biomaterials. Please feel free to contact us for more information.

Reference:

1. Golunova, A.; et al. Direct and indirect biomimetic peptide modification of alginate: Efficiency, side reactions, and cell response. International Journal of Molecular Sciences. 2021, 22(11): 5731.