Escherichia coli Lipopolysaccharide (LPS) Production Services

The Structure and Function of Lipopolysaccharides

Lipopolysaccharides (LPSs) are intricate molecules found in the outer membrane of gram-negative bacteria. Comprising lipid A, core oligosaccharide, and O-antigen or O-side chain polysaccharides, LPSs play a critical role in maintaining the integrity of bacterial membranes. The lipid A component, made up of fatty acids and glucosamine disaccharides, anchors LPS in the outer leaflet of the bacterial cell membrane. The core oligosaccharide contains various residues, including heptose, 3-deoxy-D-manno-oct-2-ulosonic acid, hexose, and hexosamine.

LPSs have significant implications for gram-negative bacterial biology and virulence, influencing the host immune system and its responses. They act as potent pyrogens and stimulators of the immune system. In severe cases, LPS can contribute to the development of septic shock. Moreover, accumulating evidence suggests that prolonged exposure to lower concentrations of LPS may have adverse effects on autoimmunity, obesity, depression, and cellular senescence.

Fig.1 Schematic of the organization of E. coli LPS. (Sondhi, et al., 2020)

E. coli LPS Production Service at CD BioGlyco

Somatic (O) or cell wall antigen, capsular (K) antigen, fimbrial (F) antigen, and flagellar (H) antigen are used to classify E. coli into 150,200 serotypes or serogroups. The most prevalent strain utilized to manufacture E. coli LPS is O antigen type E. coli. CD BioGlyco provides custom E. coli LPS production services according to the client's needs, based on the purification of LPS from specific bacteria. Besides the O antigen type, CD BioGlyco also provides the other K antigen type, F antigen type, and H antigen type. Polysaccharide antigens from microorganisms are some attractive molecules for vaccine development. CD BioGlyco provides a Carbohydrate-based Vaccine Development Platform including E. coli Polysaccharide Antigen Production Services, P. aeruginosa Polysaccharide Antigen Production Services, and other Polysaccharide Antigen Production Services.

Fig.2 Four types of E. coli. (CD BioGlyco)

Advantages of Us

• We employ an outstanding lab team that offers high-quality production outcomes.
• We provide production progress on time and make each production step clear and trackable.
• We provide different molecular weights of LPS from E. coli according to the client's needs.
• Our service team is available to respond to inquiries from clients around the world promptly.

CD BioGlyco works in offering tailored E. coli LPS production services to meet the specific needs of our clients. If you have any inquiries or require additional information, please don't hesitate to contact us. Our team is ready to assist you.

Reference:

1. Sondhi, P.; et al. Structure, formation, and biological interactions of supported lipid bilayers (SLB) incorporating lipopolysaccharide. Coatings. 2020, 10(10): 981.