Many glycans associated with cell surface receptors contain N-acetyl lactosamine (LacNAc) at the penultimate or terminal position, which regulates receptor dimerization and signaling, and is involved in mediating key cellular processes such as apoptosis, proliferation, and differentiation. CD BioGlyco uses FUTs to transfer fucose to LacNAc on the cell surface and provides professional FUTs-based cell surface chemoenzymatic glycoengineering services.
Glycosyltransferases are applied to transfer monosaccharide analogs from nucleotide sugar donors to specific glycan acceptors on the cell surface. CD BioGlyco employs a rapid and highly specific chemoenzymatic approach to perform cell surface glycoengineering services. The α1-3 fucosyltransferase (α1-3 FucT) from Helicobacter pylori has broad substrate tolerance to GDP-fucose analogs modified at the C-5 position. We use α1-3 FucT catalytic transfer of azide- or alkyne-containing fucose to LacNAc-containing cell surface glycans.
FUTs transfer monosaccharides bearing reactive groups such as azides or alkynes from a nucleotide sugar donor to a target glycan acceptor on the cell surface. CD BioGlyco uses bioorthogonal click reactions to perform click links with fluorophores or biotin probes with complementary reactive groups, thereby providing rapid and sensitive cell labeling services.
In addition, CD BioGlyco also provides glycosyl modification analysis for modified cell samples. We use a variety of technical methods, such as mass spectrometry, chromatography, and immunoassay, to conduct qualitative and quantitative glycosyl structure analysis.
Fig.1 FUTs-based cell surface chemoenzymatic glycoengineering services. (CD BioGlyco)
Technology: Bioorthogonal chemistry technology
Journal: Angewandte Chemie
IF: 16.823
Published: 2011
Results: The authors reported a specific two-step chemoenzymatic approach for site-specific chemical labeling of LacNAc-bearing glycans in living cells. The authors treated cells with non-natural monosaccharide precursors, performed fucosylation reaction under the action of α1-3 FucT, labeled the cells with azido or alkynyl groups, and then performed bioorthogonal reactions to achieve specific labeling of glycans.
Fig.2 Schematic diagram of cell surface glycan labeling process. (Zheng, et al., 2011)
CD BioGlyco provides high-quality, customized FUTs-based cell surface chemoenzymatic glycoengineering services and meets client needs in glycosyl modification and glycobiology research. Please feel free to contact us if you need chemoenzymatic glycoengineering services, we are your best partner.
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