Unraveling Complexity, One Glycosaminoglycan at a Time

Glycosaminoglycans (GAGs) are sulfated glycans in the extracellular matrix and on the cell surface that are critical for the regulation of various signaling proteins and whose function is essential in many pathophysiological systems. Therefore, GAGs can serve as useful molecular tools for researching GAG-protein interactions. At CD BioGlyco, we have constructed a variety of specific Glycan Microarrays based on the development of a comprehensive Glycan Display Platform. With our expertise in Glycan Microarray technology, we offer custom GAG microarray assays.

The GAG microarray and analysis services based on the GAG microarray we provided are as follows.

• GAG microarray construction
  • First, we prepare the GAG-protein couplers by providing either a chemoenzymatic pathway or modular assembly based on pre-synthesized building blocks.
  • Then, we dissolve the GAG-protein couplers in a spotting solution. At the same time, the insoluble material is removed by filtration.
  • Next, we covalently immobilized GAG on epoxy-activated microarray slides using a non-contact printing technique.
  • Finally, the glass slides are incubated. Subsequently, excess unimmobilized material is washed away with probe buffer and closed with buffer.
  A range of GAGs is provided in the construct, including chondroitin sulfate (CS), hyaluronic acid (HA), heparin (Hp), heparan sulfate (HS), and keratan sulfate (KS).
• GAG microarray assay
  We fluorescently label proteins coupled to GAGs. The specific binding of proteins to GAGs is detected using a fluorescence scanner. Similarly, we utilize GAG microarrays to screen for GAGs recognized by the binding proteins. In this process, we provide various binding analysis techniques to analyze the binding ability of the binding proteins to different GAGs. Such as surface plasmon resonance (SPR), pull-down analysis, flow cytometry, and so on.

Fig.1 GAG microarray assay services. (CD BioGlyco)

Publication

Applications

• GAG microarrays can be used to detect proteins or glycoproteins that bind specifically to GAGs.
• GAG microarrays can be used for high-throughput screening of GAG-mediated protein-cell interactions.
• GAG microarrays can be used to research GAG-protein interactions, leading to the preparation of GAGs that are valuable for physical and pharmaceutical research.

Advantages

• We construct GAG microarrays with high sensitivity and high throughput screening capabilities, and only a trace amount of sample is needed to complete the entire assay.
• With a professional R&D team, we provide custom glycan microarray assays according to clients' needs.
• In addition to glycan microarray assays, we also provide Microarray-based Glycoproteomics Services.

CD BioGlyco is dedicated to research in glycobiology and provides clients with advanced glycan microarray detection technologies. Currently, we have constructed a state-of-the-art glycan display platform, which covers a wide range of glycan microarrays and glycan display array-related services. Please feel free to contact us if you are interested in our services.