Gene Overexpression-based Cell Surface Glycoengineering Service
Gene Overexpression-based Cell Surface Glycoengineering Service at CD BioGlyco
Cell surface glycoengineering is a technology that studies the structure and function of cell surface sugar chains. These sugar chains are called glycoconjugates and play important roles in the physiological and pathological processes of organisms. Traditional cell surface glycoengineering technology modifies cell surface sugar chains through chemical or biological methods, but these methods have certain limitations. Gene overexpression technology studies gene function by introducing target genes into cells and causing them to be highly expressed. Cell surface glycoengineering technology based on gene overexpression has been widely used in many fields such as tumor research, immunology, and neuroscience. It has become an effective tool for studying the structure and function of cell surface sugar chains.
Fig.1 N-linked glycosylation generating biantennary sialylated glycans. (Heffner, et al., 2021)
CD BioGlyco commits to providing global clients with industry-leading Cell Surface Glycoengineering services. Our Technologies for Cell Surface Glycoengineering service provides researchers and biotech professionals with cutting-edge tools to advance research, development, and innovation in various scientific fields. Among these advanced technologies, the Genetic Cell Surface Glycoengineering Service is one of our advantageous services. The following is our gene overexpression-based cell surface glycoengineering service.
Fig.2 Service of gene overexpression-based cell surface glycoengineering. (CD BioGlyco)
Published Data
Technology: Gene Overexpression
Journal: Scientific Reports
IF: 4.997
Published: 2021
Results: The authors employed a Flp/FRT-based recombinase-mediated-cassette-exchange system to initiate the overexpression of a set of 42 human glycosyltransferase genes within Chinese hamster ovary cells. These genes participated in various stages of the core N-glycosylation pathway and exhibited diverse functionalities, encompassing tasks like sugar precursor synthesis, glycan processing, branching, galactosylation, and terminal capping. Subsequently, the authors pinpointed the pivotal genes that exerted significant influence on IgG N-glycosylation. To broaden the spectrum of complex-type N-glycan structures, these key genes were concurrently overexpressed.
Here are some of the results shown in this article for reference:
Fig.3 Impact of overexpressing individual human glycosyltransferases on growth, productivity and N-linked glycosylation of antibodies produced in stably transfected CHO cell pools. (Nguyen, et al., 2021)
Applications
- Enhanced targeting of therapeutics: Overexpression of specific glycosyltransferase genes on the cell surface leads to the increased production of specific glycan structures, such as receptor ligands or targeting molecules. This is used to enhance the targeting of therapeutic agents to specific cells or tissues.
- Vaccine production: Gene overexpression-based glycoengineering is employed in the production of glycoprotein-based vaccines. By overexpressing genes responsible for glycan synthesis, researchers ensure that the recombinant glycoproteins produced in cells carry specific glycan modifications that enhance their immunogenicity.
- Cell-based assays and drug screening: Glycoengineered cells with modified surface glycans are used in cell-based assays and drug screening studies. By overexpressing specific glycosyltransferases or glycan-modifying enzymes, researchers create cell lines that express unique glycan structures, which are used to study glycan-receptor interactions or screen for drugs that target specific glycan-related processes.
Advantages
- We employ cutting-edge methods to precisely modulate gene expression, allowing for targeted and effective cell surface glycoengineering.
- Our team collaborates closely with clients to develop customized strategies for gene overexpression, ensuring that your specific goals and applications are met effectively.
- We maintain rigorous quality control standards throughout the gene overexpression-based glycoengineering process.
With the support of the powerful Glycoengineering Platform, CD BioGlyco is dedicated to assisting clients in achieving success in the research of gene overexpression-based cell surface glycoengineering. If you have any need for gene overexpression-based cell surface glycoengineering, please feel free to contact us for more details. We also welcome inquiries regarding Gene Knockout-based Cell Surface Glycoengineering Service.
References
- Heffner, K.M.; et al. Glycoengineering of mammalian expression systems on a cellular level. Advances in Glycobiotechnology. 2021, 37-69.
- Nguyen, N.T.; et al. Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans. Scientific Reports. 2021, 11(1): 12969.
