GSL is a crucial constituent of the microdomain that offers appropriate microenvironments for the substances covering the membrane and provides support for their functions. GSL, a kind of glycolipid, is formed by a polysaccharide and a ceramide. The extensive majority of GSL structures are sorted according to seven usual tetrasaccharide neutral sugar core sequences. The structure elucidation and analysis of GSLs are fundamental steps in the functional research of GSLs.
Fig.1 GSL neutral cores and their designations. (CD BioGlyco)
CD BioGlyco utilizes LC-MS to characterize and analyze the structures of the glycan chains and lipid parts of GSLs and mixtures of GSLs. The approach is an advanced technique that combines two different processes to isolate, characterize, and analyze the number of different substances in a liquid sample.
LC is a separate step that is used to separate the pure component from a mixture. In this process, the sample is transferred by a polar mobile phase and a non-polar stationary phase. The installation is a column packed with a porous medium made of a granular solid material. That is the stationary phase where the sample is injected and the mobile phase passes to convey the sample. The sample is adsorbed on the stationary phase after being injected. These compounds are separated according to their relative affinity to the packing materials and the solvent during solvent passing. The substance is the last to separate that possesses the most affinity to the stationary phase owing to the more time high-affinity corresponding need to transfer to the end of the column.
Fig.2 LC-MS system schematic diagram. (CD BioGlyco)
When the sample is eluted by the LC column, the effluent enters into the MS. The LC-MS system possesses an ionization source where the effluent is nebulized, desolvated, and ionized to produce charged substances. These charged ions are quantified by using an electron multiplier. Our method is useful for the characterization and analysis of various GSLs. And the way displays the ability to quantify some compounds that are interesting with a high degree of sensitivity and selectivity based on the unique mass/charge transitions.
CD BioGlyco possesses matchless experience with the characterization and analysis of a wide variety of GSLs by this developed technique. We have diverse instrumentations that are state-of-the-art to help you solve problems for Carbohydrate Separation, Purification and Analysis. If you are interested in our services, please feel free to contact us for more detailed information.
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