GDP Plays a Vital Role in Cells

Guanosine 5'-diphosphate (GDP) is a nucleoside diphosphate composed of pyrophosphate unit, pentose ribose sugar, and nucleobase guanine. GDP is involved in intracellular signaling processes functioning. When an extracellular signal triggers the activation of a G protein-coupled receptor (GPCR), the associated G protein exchanges its bound GDP for GTP, activating a downstream signaling cascade, and stimulating a variety of cellular responses, containing regulated gene expression, cytoskeletal rearrangements, and modulation of enzyme activity. GTP can be hydrolyzed to GDP by the GTPase activity of the G protein, becomes inactive, and the signaling event is terminated. GDP is also a potential iron mobilizer, preventing hepcidin-ferroportin interactions. As a high-energy compound, GDP plays a vital role in cells. The mutual conversion of GDP and GTP plays a role in regulating protein activity and cell functions in cells.

Fig.1 The structure of GDP. (PubChem)

GDP Production Services at CD BioGlyco

CD BioGlyco supplies different types of GDP products, which can provide high-quality GDP in different specifications, packaging volumes, and concentrations according to custom demand.

• GDP Reagent

CD BioGlyco usually provides GDP reagents in powder or solution form, such as 5'-Guanosine diphosphate sodium salt hydrate. These reagents can be applied in experimental and study areas, such as cell signaling, and energy metabolism.

• GDP-binding Proteins

CD BioGlyco provides recombinant forms or enzyme-labeled antibodies of GDP-binding proteins, which can be used to study their functions and interactions.

• GDP Derivatives

CD BioGlyco provides some GDP derivatives that can be used for specific experimental needs, such as radioactively labeled GDP, fluorescently labeled GDP, Guanosine diphosphate mannose, Guanosine diphosphoglucose, and Guanosine diphospho-belta-L-fucose.

• GDP Detection Kits

CD BioGlyco provides kits for detecting and measuring GDP content or binding activity. These tools can be used to determine intracellular GDP levels, GTPase activity, and other related experiments.

In addition, CD BioGlyco provides customized GDP synthesis services through chemical synthesis or biosynthesis according to customer needs. Expanding the reaction system according to the scale, optimizing the process flow, and realizing efficient and controllable GDP scale-up production is also possible. Moreover, analysis and testing services are offered by CD BioGlyco, including purity analysis, structure verification, isotope labeling identification, etc.

Fig.2 Multiple types of GDP production services. (CD BioGlyco)

Applications

• RNA synthesis: GDP can participate as a substrate in the process of RNA chain elongation and repair, and can also participate in the modification reaction of specific RNA molecules.
• Protein synthesis: GDP is the precursor of GTP, which can participate in the synthesis process.
• Energy metabolism: GDP is a high-energy phosphorylation compound, GTP can provide the energy needed by cells and participate in various cellular functions and biosynthetic processes.
• Cell Signaling: GDP and related GTP are involved in activities in cell signaling pathways. As regulators of G proteins, they can transmit signals between cell surface receptors and downstream effectors, controlling the activation and inactivation of signaling pathways.
• Chemical and biology research: GDP and its derivatives can be used in chemical and biology studies, for example, the design and development of GTPase regulators with specific functions, enzymatic reactions, and drug screening.

Advantages

• CD BioGlyco has advanced laboratory facilities and production equipment to support high-quality CDP production.
• CD BioGlyco has advanced quality control and analysis methods to ensure that CDP products meet specifications and standards.
• CD BioGlyco will quickly respond to customer needs and deliver high-quality CDP products on time.

CD BioGlyco provides a full range of GDP services and products. Other Nucleotide-based Production Services are also offered, including Adenosine Diphosphate (ADP), Cytidine Diphosphate (CDP), and Uridine Diphosphate (UDP). Please feel free to contact us as soon as possible if you are interested in our production services.