Hibiscus Mutabilis Seed Lectin Production Service
Hibiscus mutabilis Seed Lectin Production Service at CD BioGlyco
CD BioGlyco is equipped with a specialized research team and state-of-the-art technology for Plant Lectin Production and testing H. mutabilis is a deciduous shrub. The researchers extracted a lectin from the seeds that has strong HIV-1 reverse transcriptase inhibitory activity. This discovery also provides data to support the application of H. mutabilis. To fulfill our clients' research needs for H. mutabilis seed lectins, we have developed a one-stop Lectin Production service.
We extract lectins from H. mutabilis seeds. We optimize the reported extraction method to avoid the problems of product impurity, long experimental period, etc., encountered in traditional lectin production. We then use a combination of gel filtration chromatography and ion exchange chromatography to further purify the crude extract from the seeds. After the purity and content test, the optimized production method improves the yield and purity of lectin.
Upon the client's request, we test the physicochemical properties of the extracted lectin. We detect its molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The lectin extracted from H. mutabilis seeds is found to have binding specificity for galacturonic acid. We test the inhibitory effect of metal ions and different kinds of carbohydrates on their agglutination activity. In addition to this, agglutination activity and others are also tested.
Fig.1 Process of H. mutabilis seed lectins production. (CD BioGlyco)
Publication
Technology: Ion Exchange Chromatography and Gel Filtration Chromatography
Journal: Acta Biochimica Polonica
IF: 1.42
Published: 2009
Results: In this study, a lectin was extracted from dried H. mutabilis seeds. It was purified using a combination of gel filtration chromatography and ion exchange chromatography. It was found to have a molecular weight of 150-kDa by SDS-PAGE. the results of sugar inhibition experiments showed that the lectin had binding specificity for galacturonic acid. It also exhibited strong HIV-1 reverse transcriptase inhibitory activity and weak antiproliferative activity against tumor cells. However, it showed no inhibitory activity against Fusarium oxysporum, Mycosphaerella arachidicola, Valsa mali, etc.
Fig.2 Purification of H. mutabilis lectin. (Lam & Ng, 2009)
Applications of H. mutabilis Seed Lectin Production
- H. mutabilis seed lectins produced by our optimized method are used to develop new lectin staining techniques based on lectin-glycan interactions.
- H. mutabilis seed lectins produced by our optimized method are used for screening studies of disease-related markers.
- H. mutabilis seed lectins produced by our optimized method are used for affinity chromatography.
Highlights of Us
- With our optimized production method, we maximize the yield and purity of H. mutabilis seed lectin.
- Our professional lectin research team develops exclusive experimental protocols for clients' needs and also updates the progress of experiments in time.
CD BioGlyco is constantly improving its production capacity. It is our goal to provide our clients with efficient and short lead-time lectin production services. In addition to H. mutabilis seed lectin, we also provide Griffonia simplicifolia Lectin Production services, etc. Please feel free to contact us for detailed information about the detailed steps of lectin production and purification. We are honored to serve you.
Reference
- Lam, S.K.; Ng, T.B. Novel galactonic acid-binding hexameric lectin from Hibiscus mutabilis seeds with antiproliferative and potent HIV-1 reverse transcriptase inhibitory activities. Acta Biochim Pol. 2009, 56(4): 649-654.
