High-Throughput Screening (HTS) of Glycan from Glycoengineered Cell Line (GCL)

Glycoengineering is the technique that modifies glycosylation modifications in organisms, which controls the type and extent of glycosylation modifications by transforming expression to inhibit or promote glycosylation enzymes. GCLs are cell lines that are genetically engineered to modify cells to have specific glycosylated structures and glycan metabolism functions. GCLs are advantageous in terms of glycosylation modifications because of the efficient glycosylation mechanisms in these cells that modify them during protein expression. Currently, researchers have successfully screened many glycans with biological functions or pharmacological effects from GCL by HTS techniques. Among these techniques, chemical synthesis, biosynthesis, and synthetic biology provide a wide range of options for the HTS of glycans. Overall, the continuous HTS technology provides more possibilities for glycan discovery and application in GCL, which will provide breakthroughs and opportunities for the development and application of human life sciences.

Fig.1 Overview of glycoengineering strategies. (Narimatsu, et al., 2021)

High-Throughput Glycan Screening for Glycoengineered Cell Lines at CD BioGlyco

HTS of glycans for GCL helps scientists better understand the role of glycans in biology, as well as develop new drugs and therapeutics. CD BioGlyco is equipped with a team of professionals who have extensive experience in HTS and we provide the most reliable and sophisticated technologies and complete customized after-sales service. The high-throughput glycan screening services for GCL we provide include but are not limited to:

• High-throughput glycan screening for GCL by Ultra-High Performance Liquid Chromatography-Fluorescence Detector (UHPLC-FLD)
  We not only detect individual glycan molecules but also analyze information such as glycan chain structure and glycan chain length, which provide important references in your glycobiology research. High-throughput glycan screening by this equipment is fast, high throughput, high sensitivity, and high resolution.
• High-throughput glycan screening for GCL by Capillary Electrophoresis-Mass Spectrometry (CE-MS)
  This technology enables rapid and accurate identification and quantification of glycans by analyzing their different mobile properties in mass-to-charge ratio. We simultaneous processing of a large number of samples per analysis, resulting in high efficiency and speed of experiments.

Fig.2 Technologies for high-throughput glycan screening for GCL. (CD BioGlyco)

Applications

• Glycan-based vaccine development
• Research on glycomics
• Carrier or auxiliary substance for synthesized drugs

Advantages of Us

• Our technology can analyze various types of sugars
• High-throughput autosampler and separation columns for rapid screening of several glycan molecules
• Forward-looking services

CD BioGlyco offers the highest quality services to our clients, utilizing the latest technologies and complete solutions to help them promote their research. We offer professional high-throughput glycan screening services for GCL and perfect after-sales service. If you are interested in our services, please contact us for more details.