Overview of Glyconanoparticle in Agriculture

Insecticides serve as a critical material foundation for defending against major agricultural pests and diseases, ensuring food security and agricultural productivity. Despite their importance, the effectiveness and safety of insecticides need further enhancement, largely due to factors such as wind, sunlight, rain, and pest resistance. The declining efficiency of insecticide use has led to increased formulations and crop losses, posing significant challenges to the sustainable control of pests and pathogens. Nonetheless, insecticides remain one of the most effective methods for controlling most plant diseases, providing indispensable benefits to crop production. Thus, enhancing the effectiveness and safety of chemical pesticides is essential for efficient pest management. Drawing from our extensive experience, CD BioGlyco provides insecticide-loaded glyconanoparticle formulation to improve utilization in the use of pesticide mixtures, which can reduce unnecessary large-scale and repetitive applications.

Smart Nanotech Formulations, Safeguarding the Harvest Path

Through our GlycoNano™ Platform, we utilize chitosan-based nanoparticles as carriers, which offer unique advantages such as high stability, biocompatibility, and the ability to enhance the efficacy of insecticides. These nanoparticles are specifically designed to encapsulate and deliver insecticides with precision, targeting harmful insects while minimizing environmental impact.

Synthesis of Insecticide-Loaded Chitosan Nanoparticles

We dissolve chitosan powder in an acetic acid solution with constant stirring using a magnetic stirrer. A solution of tripolyphosphate (TPP) containing the agrochemicals (spinosad and permethrin) is prepared. Add the agrochemical-TPP solution to the chitosan solution and stir continuously to form the nanoparticles. The suspension is centrifuged to separate the nanoparticles, which are then lyophilized to obtain the final product.

Characterization of Chitosan and Agrochemical Loaded Chitosan Nanoparticles

• We disperse the nanoparticles in water and analyze them using a UV-Vis spectrophotometer to confirm their formation and assess loading efficiency.
• By analyzing dried powdered samples through X-ray diffraction (XRD), we determine the crystalline structure and phase composition of the nanoparticles.
• Fourier Transform Infrared Spectroscopy (FT-IR) is used to obtain the infrared spectrum of the nanoparticles enabling us to identify functional groups and ensure the integrity of the chemical encapsulation.
• Field emission scanning electron microscopy (FE-SEM) is utilized to examine the surface morphology and particle size of the nanoparticles in detail.
• High-resolution transmission electron microscopy (HR-TEM) provides detailed structural information and verifies the uniformity of the nanoparticles.
• We disperse the nanoparticles in a solvent and analyze them to measure size distribution and surface charge, which are crucial for their stability and dispersion characteristics.

Analysis of Loading Efficiency

First, a chitosan solution is prepared by dissolving it in acetic acid with continuous stirring. Simultaneously, a solution of TPP containing the agrochemicals is prepared. The agrochemical-TPP solution is then added dropwise to the chitosan solution while stirring continuously to facilitate cross-linking. After mixing, the mixture is centrifuged to separate the nanoparticles, and the collected supernatant is analyzed using a UV-Vis spectrophotometer to measure its optical density (OD). This measurement is crucial for calculating the loading efficiency of the agrochemicals, and determining how much of the active ingredient is successfully encapsulated within the nanoparticles.

Release Rate Studies

The agrochemical-loaded chitosan nanoparticles are initially suspended in a Tris-HCl buffer solution. This suspension is then stirred continuously at a controlled temperature, and at specified intervals, the sample is centrifuged to separate the supernatant. The amount of agrochemicals released into the supernatant is subsequently measured using a UV-Vis spectrophotometer, providing critical data on the release profile and efficiency of the encapsulation process.

Biological Activity Analysis

We prepare chitosan and agrochemical-loaded chitosan nanoparticles, which we then apply to food vials to treat Drosophila melanogaster (fruit flies) in various assays. These assays include survivability, where we observe the number of eggs laid and hatched; climbing, where we measure the upward movement of the flies; and larval crawling, where we record the behavior and speed of third-instar larvae. We perform the experiments in triplicates and repeat them multiple times, calculating mean values and standard deviations. We use one-way ANOVA for statistical analysis to determine the significance of the results compared to control values. The overall aim of our work is to evaluate the effects of the nanoparticles on the survival, mobility, and behavior of fruit flies, providing insights into the potential impact of the agrochemical encapsulation on larval development and adult performance.

Workflow

Publication Data

Applications

• Our service can be used to optimize resource utilization in precision agriculture for maximal yield.
• Our service is applied to developing highly efficient, and low-toxicity pesticides, providing more innovative solutions for the agricultural companies..
• Our service is useful for research to reduce pesticide residues on crops, enhancing food safety.

Advantages

• We encapsulate and deliver insecticides with precision, targeting harmful insects while minimizing environmental impact.
• Our formulations undergo rigorous field trials to validate their efficacy under real-world conditions.
• We offer customized glyconanoparticle formulations tailored to the specific pest control needs of different crops and regions.

Frequently Asked Questions

• Which insecticides can we load into glyconanoparticles?

  Insecticide-loaded glyconanoparticle formulation
  Chlorpyrifos	Hexazole alcohol	Glyphosate
  Cypermethrin	Avermectin	Imidacloprid
  Trichlorfon	Pyrethrin	Citronella
  2,4-Dsodiumsalt	Lambda Cyhalothrin	Permethrin
  Spinosad	Rotenone	Acetamiprid

• What are the reasons for selecting nanomaterials as carriers?
  Nanomaterials, characterized by their small size, large specific surface area, and modifiability, significantly enhance the adhesion of pesticides to both leaf surfaces and harmful target surfaces. This enhancement leads to improved efficiency by reducing the wastage attributable to coarse particles and imprecise release mechanisms. Additionally, it boosts the biological activity and overall efficacy of the pesticides.

CD BioGlyco offers diverse Glyconanoparticle Formulation Services and provides a comprehensive approach to the development and characterization of advanced insecticide-loaded glyconanoparticles, ensuring their effectiveness and safety in agricultural applications. If you are committed to enhancing overall ecosystem well-being and backing more sustainable agricultural methods, contact us today!