Maleimide-Thiol Coupling-based Cell Surface Chemical Conjugation Glycoengineering Service

Maleimide-Thiol Reaction of Cell Surface Glycans

The set of glycans displayed on the cell surface determines how that cell interacts with other cells and molecules in its environment. Thus, methods for controlling the expression of glycans are valuable tools for understanding and regulating the biological functions of cell surface glycoconjugates. Among the tools for controlling the expression of glycans, coupling reactions have a unique function. With the development of coupling technology, a large number of new coupling strategies have been developed. The maleimide-thiol reaction, a payload loading approach, is better controlled than the classical coupling reaction. The reagent improves the reactivity without in situ activation. The reaction is now widely used in the production of protein-polymer couplings with powerful therapeutic properties. It also exhibits high stability, high water solubility, and site specificity.

Fig.1 Cell surface display of defined glycans reveals glycan function and dictates cell fate. (Nischan & Kohler, 2016)

Maleimide-Thiol Coupling-based Cell Surface Chemical Conjugation Glycoengineering Service at CD BioGlyco

CD BioGlyco is dedicated to Glycoengineering Platforms where we combine chemical and biological approaches for the development of Cell Surface Glycoengineering Techniques. In chemical conjugation-based cell surface glycoengineering techniques, we provide maleimide-thiol coupling-based cell surface chemically conjugated glycoengineering services.

• We conjugate the maleimide derivatives of glycans to cell surface thiols through Michael's addition reactions to form stable thioether-linked structures.
• We utilize single molecule force spectroscopy to determine the stability of the thioether bond in the maleimide-thiol coupling.
• We utilize kinetic control experiments to assist in the production of stable glycan-protein concatenates.
• We utilize different molar ratios and reaction times of maleimide to thiols and quantify unbound thiols by liquid chromatography to determine the efficiency of cell surface protein concatenation.
• We determine the modification sites obtained after coupling reactions of glycans by fluorescent labeling and other related means.
• We utilize chemical means to detect, image, and purify specific groups of glycan affixes.

Fig.2 Based on maleimide-thiol coupling for glycoengineering services. (CD BioGlyco)

Applications

• Maleimide-thiol coupling-based cell surface chemical conjugation glycoengineering can be used to research molecular interactions and biological roles of glycans, e.g. glycan biorecognition, and glycan-protein coupling.
• Maleimide-thiol coupling-based cell surface chemical conjugation glycoengineering can be used for in-depth research on the processing of glycan analogs of cell surface glycans.
• Maleimide-thiol coupling-based cell surface chemical conjugation glycoengineering can be used in research to create coupling reactions for specific biofixes.

Advantages

• We have a specialized, systematic glycoengineering platform with world-leading glycan biology technologies.
• Our chemical conjugation-based cell surface glycoengineering service contains a wide range of chemo-biological tools for our clients to choose from.
• Our service team is highly sensitive and specialized to provide custom research solutions to each client.

CD BioGlyco relies on specialized experimental equipment to establish a powerful glycoengineering platform. Our platform contains rich glycoengineering techniques, such as the cell surface glycoengineering technique. We do our best to help our clients solve the difficult problems in glycan biology, and we hope to be the right hand of our clients. If you are interested in our services, please feel free to contact us.