α-Mannose, Lewis a (Lea) & Lewis x (Lex)-binding Lectin Production Service
Lectins are categorized into natural lectins and recombinant lectins according to the type of production. CD BioGlyco offers comprehensive Lectin Production Services with state-of-the-art production technology. For the production of α-mannose, Lewis a (Lea) & Lewis x (Lex)-binding lectins, we offer recombinant lectins, purification, and characterization services.
Firstly, we identify the gene encoding α-mannose lectin by bioprobe technology. Next, the α-mannose lectin precursor is encoded in specific cells to bind to the ends of Lewis a (Lea) & Lewis x (Lex) residues to obtain recombinant lectins.
We purify using Affinity Chromatography. Then, gel filtration is used and the eluate is collected. Finally, the eluate is concentrated to obtain the lectin.
- Characterization of lectins
- Glycan binding specificity
We characterize the lectin using glycan-splicing microarrays and affinity chromatography to obtain the glycan-binding capacity of the lectin.
- Molecular weight
We use mass spectrometry and gel filtration chromatography to determine the molecular weight of this recombinant lectin.
- Hemagglutination activity
The hemagglutination activity of the recombinant lectin is determined by the hemagglutination of animal cells, bacteria, and viruses.
Fig.1 Recombinate lectin production services. (CD BioGlyco)
Publication
- Technology: Lectins were reconstituted and characterized using chromatography and glycan arrays.
- Journal: Immunobiology
- IF: 2.8
- Published: 2016
- Results: Aiming to further research the role of mannose-binding lectin (MBL) in chickens, recombinant chicken MBL (RcMBL) was produced in HeLa R19 cells and purified using mannan affinity chromatography followed by gel filtration. Upon structural testing, it was found that RcMBL was structurally and functionally similar to natural chicken MBL (NcMBL) isolated from serum. RcMBL was expressed as an oligomeric protein with a monomer mass of 26 kDa as determined by mass spectrometry. Glycan array analysis showed that RcMBL was most strongly bound to high-mannose glycans in the cell, but was most strongly bound to the glycans with terminal fucose and GlcNac residues. Next, it was demonstrated that RcMBL was biologically active by its hemagglutination activity for the agglutination of Salmonella typhimurium and the inhibition of influenza A virus.
Fig.2 Western blotting analysis of RcMBL and NcMBL. (Zhang, et al., 2016)
Applications
- α -Mannose, Lewis a (Lea) & Lewis x (Lex)-binding lectins are used for the analysis of glycoproteins and glycosylation.
- The α -Mannose, Lewis a (Lea) & Lewis x (Lex)-binding lectin is used for efficient and selective separation of glycosylated virus particles and glycoproteins.
Advantages of Us
- Our products pass quality tests to ensure that we fulfill our clients' research requirements.
- Our professional R&D team will customize production solutions according to the client's requirements, and our goal is to meet clients' needs beyond expectations.
CD BioGlyco has advanced technology in the field of glycobiology and we have developed several types of lectin production services. For example, Mannan-binding Lectin. We will continue to optimize our experimental protocols according to the needs of our clients to ensure their satisfaction. Please feel free to contact us at any time if there is any need.
References
- Zhang, W.D.; et al. Expression and characterization of recombinant chicken mannose-binding lectin. Immunobiology. 2016, 222(3): 518-528.
- Sato, T.; et al. Engineering of recombinant Wisteria floribunda agglutinin specifically binding to GalNAcβ1, 4GlcNAc (LacdiNAc). Glycobiology. 2017, 27(8): 743-754.
This service is for Research Use Only, not intended for any clinical use.
