Mechanism of N-glycosylation

Protein glycosylation affects protein features, for example, protein folding, stability, and degradation. There exist two types of glycosylation, containing N-glycosylation and O-glycosylation. N-glycosylated secretory proteins within the eukaryotic endoplasmic reticulum and Golgi complex, N-glycans are transferred via oligosaccharyltransferase, it is an endoplasmic reticulum-integrated membrane enzyme. When glycoproteins are transported to their final destination, they are further modified in the Golgi complex to form multiple structures with physiological functions. By regulating the glycosyl structure and modification pattern of N-glycosylated proteins-based cell surface, the type, position, and quantity of glycosyl groups are changed, thereby precisely regulating their activity and interaction.

Various Types of N-glycoprotein-based Cell Surface Glycoengineering Services at CD BioGlyco

CD BioGlyco has a leading-edge technology expression platform with proven experience in glycoengineering, which offers various N-glycoprotein-based cell surface glycoengineering services.

• Glycosyltransferase enzyme assay

CD BioGlyco provides mature enzymatic analysis services to test the activity and substrate specificity of specific glycosyltransferases in cells.

• Gene engineering

The genes related to N-glycoprotein are introduced or silenced to change the sugar structure-based cell surface by genetic manipulation.

• Cell culture and biosynthesis

CD BioGlyco provides services to optimize cell culture conditions and biosynthetic pathways to achieve specific glycosylation patterns.

• Analysis and evaluation

CD BioGlyco provides qualitative and quantitative analysis of N-glycoprotein-based cell surfaces using mass spectrometry (MS), nuclear magnetic resonance (NMR), and other techniques to evaluate the modification effect and determine the structural characteristics of glycosylation. For example, combining metabolic labeling, copper-free click chemistry, enzymatic reaction, and MS-based proteomics enables site-specific analysis of cell surface N-glycoproteins.

Glycosylation modifications of cell surface glycoproteins have a noticeable impact on functions and interactions. The effects of different glycosylation models on protein function, signaling, and interactions may be researched by an N-glycoprotein-based cell surface glycoengineering service.

Fig.1 N-glycoprotein-based cell surface glycoengineering services. (CD BioGlyco)

Applications

• Disease research: Abnormal glycosylation is interrelated to the occurrence of diseases. Utilizing the N-glycoprotein-based cell surface glycoengineering service, the state of disease-related glycosylation modifications is simulated and regulated, and its association with the pathogenesis of diseases is studied.
• Vaccine development: The N-glycoprotein glycoengineering services may transform the glycosylation pattern-based cell surface glycoproteins, and combine specific antigenic peptides with glycosylation modifications, which may improve the antigenicity and immunogenicity of vaccines.
• Drug development: N-glycoprotein-based cell surface plays a vital role in drug target recognition, drug delivery, and immunotherapy.

Advantages

• CD BioGlyco provides a variety of N-glycoprotein-based cell surface sugar modification methods and technologies to meet different purposes.
• CD BioGlyco has the experience and ability to modify N-glycoprotein-based surface sugars on cells of different origins and types.
• CD BioGlyco has established a complete quality management system to ensure the accuracy and reliability of N-glycoprotein-based cell surface sugar modification results.

CD BioGlyco provides high-level professional N-glycoprotein-based cell surface glycoengineering services. Moreover, we also provide other glycoengineering services including Cell Surface N-glycan Glycoengineering Service, Cell Surface O-glycan Glycoengineering Service, and Cell Surface Glycosylphosphatidylinositol (GPI) Anchor Glycoengineering Service. Please feel free to contact us in time if you would like to acquire related glycoengineering services.