Cell Surface N-glycan Glycoengineering Service

Cell Surface N-glycan Glycoengineering Service at CD BioGlyco

Cell surface N-glycans are complex carbohydrate structures that play critical roles in a variety of cellular processes, including cell signaling, adhesion, and interactions with other cells or molecules. Modification of these cell surface N-glycans is of great interest in both basic research and biotechnological applications. Engineering of cell surface N-glycans allows precise control of glycan structure, thereby affecting cell behavior and function.

Fig.1 Cell-surface N-glycan editing in a subtype-selective manner. (Tang, et al., 2022)

At CD BioGlyco, the Cell Surface Glycoengineering Service aims to provide a platform for the modification and engineering of cell surface glycans. Cell surface glycoengineering service contains many types of sub-services, among which, cell surface N-glycan glycoengineering service is an emerging technology that modulates the biological behavior and function of cells by changing the structure and composition of cell surface N-glycans. Our cell surface N-glycan engineering service encompasses a range of techniques and tools for the manipulation of N-glycan structures on the cell surface. This includes but is not limited to:

• Identification of N-glycan structure: Use various sugar chain analysis techniques, such as mass spectrometry, nuclear magnetic resonance, etc., to identify the structure and composition of N-glycans on the cell surface, and clarify the types of N-glycans and modification sites to be modified.
• Design a genetic modification plan: According to the type of N-glycan to be modified and the modification site, design a genetic modification plan, including inserting, knocking out, or mutating relevant genes, to achieve modification of the N-glycan structure.
• Construct a gene expression vector: Construct the modified gene into the expression vector to achieve high expression of the target gene.
• Transfection/infection of cells: Use transfection/infection technology to introduce gene expression vectors into target cells.
• Cell culture and screening: Carry out in vitro culture and conduct necessary molecular biology and cell biology experiments to verify the efficiency and specificity of genetic modification.
• Detect changes in cell surface N-glycans: Use various sugar chain analysis techniques to detect changes in cell surface N-glycan structures after genetic modification.
• Detect changes in cell biological behavior: Use cytology and molecular biology experiments to detect the impact of genetic modification on cell biological behavior, including cell proliferation, apoptosis, migration, differentiation, etc.

Fig.2 Content of cell surface N-glycan engineering service. (CD BioGlyco)

CD BioGlyco utilizes this cutting-edge biotechnology to provide high-quality cell surface N-glycan glycoengineering service. Our suite of services allows precise modification of various types of cell surface N-glycans to meet clients' research and biotech needs.

Applications

• Biopharmaceutical production optimization: Glycoengineering cell surfaces for enhanced N-glycan profiles is crucial in biopharmaceutical production. It enables the development of cell lines that produce therapeutic glycoproteins with improved stability, efficacy, and reduced immunogenicity. This is particularly important in the production of monoclonal antibodies and other biologics.
• Cancer research and immunotherapy: Glycoengineering cell surfaces can aid in cancer research by studying how altered N-glycans on cancer cells influence metastasis, immune evasion, and cancer progression. Moreover, it has the potential to develop glycoengineered immune cells for cancer immunotherapies, boosting the body's ability to recognize and target cancer cells.
• Stem cell engineering and regenerative medicine: Cell surface N-glycan glycoengineering can be applied to stem cells, allowing the creation of glycoengineered stem cells with specific glycan profiles. These engineered cells can be used in regenerative medicine and tissue engineering, potentially improving cell therapies and organ regeneration.
• Glycan-mediated cell signaling studies: This service facilitates the study of glycan-mediated cell signaling. By modifying the N-glycans on cell surfaces, researchers can investigate how these changes affect cell adhesion, signal transduction, and immune response. This information is valuable for understanding disease mechanisms and developing new therapeutic strategies.

Advantages

• With years of experience in the field, we bring advanced knowledge of N-glycan glycoengineering to the table.
• We offer customized services to meet your specific research goals.
• We have established a high-quality Glycoengineering Platform.
• We have rigorous quality control measures in place to guarantee the consistency and reliability of our services.

CD BioGlyco provides systematic overall solutions based on Cell Surface Glycoengineering. Our cell surface N-glycan glycoengineering service allows precise modification of various types of cell surface N-glycans to meet your research and biotech needs. If you are interested in our services, please do not hesitate to contact us for more details.