Protein N-glycosylation plays an important role in pathological and physiological processes. Characterizing N-glycosylation becomes extremely necessary in protein research. CD BioGlyco has excellent technology platforms and unique database to meet the customer’s research needs and obtain reliable results. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Glycosylation is one of the most common post-translational modifications spanning more than 50% of the proteome, and its role in diseases such as cancer, infectious diseases and cardiovascular diseases has been confirmed in numerous studies. Glycan microheterogeneity (site-specific glycan structure) and macroheterogeneity (glycosylation site occupancy) form the integral heterogeneity of proteins which is essential to understand the biosynthesis and function of glycoprotein. N-glycosylation site occupancy reflects the proportion of N-glycosylation in the protein, and this phenomenon in turn reveals the availability of sites, substrate concentrations and enzyme kinetics in ER. The properties of glycoprotein are largely affected by the site occupancy, and defects in site occupancy may be detrimental to physiological function.

Fig 1. Representative spectra of H1 glycopeptide. (Cruz, 2017)

Services

N-glycosylation site occupation analysis in CD BioGlyco including but is not limited to the following technologies.

Liquid chromatograph mass spectrometry ((LC-MS) based quantification has become widely used and considerably advanced quantification of N-glycosylation site occupancy in samples which labeling and label-free methods are frequently used. SILAC, TMT, dimethylation and iTRAQ are commonly used to introduce ‘heavy’ and ‘light’ isotopes or isobaric tags into samples while label-free methods are based on spectral counting or peptide-ion intensity.

• Labeling methods: fold change of the deglycosylated peptide to the total protein levels or the average of all non-glycosylated peptides.
• Label-free methods: the abundance of the deglycosylated peptide as a fraction of the sum of the deglycosylated peptide and unmodified versions of the same peptide.
• Multiple Reaction Monitoring (MRM) methods: the application of selected reaction monitoring to multiple product ions from one or more precursor ions.

Applications

• Molecular & Cellular proteomics
• Glycoprotein biosynthesis and function
• Disease pathology and progression
• Disease diagnosis and treatment
• Development of new biological drugs

Advantages of Us

• Provide a variety of markers
• Multiple technology platforms
• Varied fragmentation modes
• Strict quality control management
• One-stop services

CD BioGlyco provides diverse and systematic glycosylation site occupation analysis services. The efficient services and accurate results have gained a good reputation worldwide for our company. We will continue to improve the quality of services and bring convenience to customers' glycobiology research in order to save time and funds for customers while obtaining credible results.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

Reference:

1. Cruz, E.; et al. Site-specific glycosylation profile of influenza A (H1N1) hemagglutinin through tandem mass spectrometry. Human vaccines & immunotherapeutics. 2017, 0: 1-10.