Protein N-glycosylation plays an important role in pathological and physiological processes. Characterizing N-glycosylation becomes extremely necessary in protein research. CD BioGlyco has excellent technology platforms and unique database to meet the customer's research needs and obtain reliable results. We have confidence to be your essential research assistant in the field of glycobiology.

Background

Glycosylation is one of the most common post-translational modifications spanning more than 50% of the proteome, and its role in diseases such as cancer, infectious diseases and cardiovascular diseases has been confirmed in numerous studies. Glycan microheterogeneity (site-specific glycan structure) and macroheterogeneity (glycosylation site occupancy) form the integral heterogeneity of proteins which is essential to understand the biosynthesis and function of glycoprotein. N-glycosylation site occupancy reflects the proportion of N-glycosylation in the protein, and this phenomenon in turn reveals the availability of sites, substrate concentrations and enzyme kinetics in ER. The properties of glycoprotein are largely affected by the site occupancy, and defects in site occupancy may be detrimental to physiological function.

Technologies

N-glycosylation site occupation analysis in CD BioGlyco, including but not limited to the following technologies.

Liquid chromatograph mass spectrometry (LC-MS) based quantification has become widely used and considerably advanced quantification of N-glycosylation site occupancy in samples which labeling and label-free methods are frequently used. Stable isotope labeling by amino acids in cell culture (SILAC), tandem mass tag (TMT), and dimethylation are commonly used to introduce 'heavy' and 'light' isotopes or isobaric tags into samples, while label-free methods are based on spectral counting or peptide-ion intensity.

• Labeling methods: Fold change of the deglycosylated peptide to the total protein levels or the average of all non-glycosylated peptides.
• Label-free methods: The abundance of the deglycosylated peptide as a fraction of the sum of the deglycosylated peptide and unmodified versions of the same peptide.
• Multiple reaction monitoring (MRM) methods: The application of selected reaction monitoring to multiple product ions from one or more precursor ions.

In-depth Analysis of N-Glycosylation Sites

Our company adopts matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) technologies, combined with bioinformatics algorithms, to achieve highly sensitive localization of glycoproteins' N-glycosylation sites, quantification of site occupancy, and structural analysis of the glycan, and to facilitate the study of glycobiological mechanisms.

• N-glycosylation site analysis: Our researchers provide all N-glycosylation site identification services in proteins to help clients determine the glycosylation status (glycosylated or unglycosylated) of each site.
• N-glycosylation site occupancy analysis: We provide N-glycosylation site occupancy analysis, such as the relative proportion of glycosylation sites, by labeling or quantitative MS.

Workflow

Applications

• Our services can be used for biopharmaceutical quality control. Not only can it be used for glycosylation site analysis of biopharmaceuticals to ensure product consistency and stability. It can also be used to assess the occupancy of glycosylation sites and optimize the production process.
• Our services can be used for systematic studies of glycoproteins to reveal the distribution and function of glycosylation sites.
• Our services can be used for heterogeneity analysis of glycosylation sites to explore their role in disease.
• Our services can be used to evaluate the effect of glycosylation on drug activity, immunogenicity, and half-life.

Advantages of Us

• Our service is applicable to complex glycoproteins with multiple glycosylation sites and also supports the analysis of non-standard glycosylation sites.
• We use specialized enrichment strategies to improve the detection rate of N-glycosylation sites.
• Our company provides one-stop service, from sample processing to data analysis, and the whole process is operated by a professional team.

Publication Data

Frequently Asked Questions

CD BioGlyco provides diverse and systematic glycosylation site occupation analysis services. The efficient services and accurate results have gained a good reputation worldwide for our company. We will continue to improve the quality of services and bring convenience to customers' glycobiology research in order to save time and funds for customers while obtaining credible results.

Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.

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