Narcissus pseudonarcissus Lectin (NPL) Production Service at CD BioGlyco

CD BioGlyco is a trusted supplier in the field of Lectin Production and distribution. Researchers isolated NPL from N. pseudonarcissus bulbs. It is specific for α-linked mannose and prefers polymannose structures containing (α-1,6) bonds. Unlike, for example, Canavalia ensiformis lectin, NPL does not bind glucose. The production and biochemical characterization of NPL is necessary for its application research. We offer one-stop NPL production services and a wide range of NPL products to meet our clients' needs for different Plant Lectin Production.

Fig.1 Process of NPL production. (CD BioGlyco)

• NPL production service

NPL exists as a dimer. It is currently extracted from both bulbs and leaves of N. pseudonarcissus. We optimize the reported extraction methods using both bulbs and leaves. Buffer-soaked extraction is followed by precipitation through ammonium sulfate. To obtain high-purity NPL, we use a combination of Ion Exchange Chromatography and Affinity Chromatography for purification. After purification, pure NPL is finally obtained by freeze-drying.

NPL is an unglycosylated homodimer with a molecular weight of 26 kDa. We develop customized biochemical characterization protocols. Molecular mass, hemagglutination activity, thermal stability, sugar-binding specificity, and amino acid composition are measured. We also use circular dichroism to determine the secondary structure of NPL.

We also produce a variety of labeled conjugate products using purified NPL, including peroxidase conjugate, agarose conjugate, colloidal gold conjugate, and others.

• NPL products

The use of optimized methods is validated to increase NPL yield and purity while reducing production cycle time. We have a wide range of NPL products and are capable of meeting the different needs of our clients for various experiments on lectin products.

Applications of NPL Production

• NPL is found to have insecticidal properties. The NPL we produce is used in the development of insecticides.
• Our NPL is used for affinity chromatography purification of N-linked glycoconjugates.
• Our NPL is used to label glycoconjugates present on the cell surface, allowing visual localization of glycoconjugates on the cell surface.

Advantages of Us

• We contain unlabeled and labeled NPL products. Fluorescein, biotin, rhodamine, CY3, and many other markers are available.
• We are passionate, forward-thinking, and committed to providing our clients with world-class lectin production services and a wide range of lectin products.

CD BioGlyco has an experienced and highly qualified product manufacturing team ready to provide a wide range of services to our clients. We always put our clients' needs first. In addition to NPL, we also offer production for Mangifera indica Lectin, Musa paradisiaca Lectin, and more. If you are interested in our customized lectin production service and various related products, please feel free to contact us to inquire about the detailed experimental procedure, price, and so on. We are honored to serve you.

Reference:

1. Summers, C.; et al. The potentially insecticidal Narcissus pseudonarcissus lectin demonstrates age-related mitogenicity. FEMS Immunology & Medical Microbiology. 2002, 33(1): 47-49.