Canavalia Ensiformis Lectin Production Service

Canavalia Ensiformis Lectin Production Service

Canavalia ensiformis Lectin Production Service at CD BioGlyco

CD BioGlyco is ready to serve clients with high-quality Lectin Production Services at any time. Plant Lectins are a class of sugar-specific proteins or glycoproteins that promote cellular agglutination. They have been widely studied and produced. C. ensiformis lectins is found to bind specifically to D-mannose or D-glucose. Concanavalin A (Con A) extracted from C. ensiformis is one of the most widely used lectins. It is used in several biochemical research techniques. Based on its good application prospects, We provide a one-stop service for C. ensiformis lectin production.

Fig.1 Process for the production of C. ensiformis lectin. (CD BioGlyco)Fig.1 Process for the production of C. ensiformis lectin. (CD BioGlyco)

  • C. ensiformis lectin production service

Lectins are highly abundant in C. ensiformis seeds. We mainly extract lectins from seed species. Using C. ensiformis seeds as raw material, we summarize the published extraction process and optimize it. The optimized extraction method greatly shortens the experimental period.

Most lectins belong to proteins, so many protein purification methods are used to purify lectins. There are many methods for lectin isolation and purification, mainly including ion-exchange chromatography, Gel-filtration Chromatography, affinity chromatography, and other methods. We purify C. ensiformis lectin by Ion Exchange Chromatography and Affinity Chromatography. C. ensiformis lectin is heat stable. We compare the hemagglutination activity of lectins obtained by extraction at different temperatures to determine the optimal temperature for purification. The optimized method greatly improves efficiency.

After purification, we study the physicochemical properties of C. ensiformis lectin with high-performance liquid chromatography, gas chromatography-mass spectrometry, and other instruments.

  • C. ensiformis lectin products

We maximize the yield and purity of C. ensiformis lectin through optimized extraction and purification methods. We have a wide range of C. ensiformis lectin products, including Con A, biotin conjugate, FITC conjugate, etc.

Publication

Technology: Affinity Chromatography

Journal: International Journal of Biological Macromolecules

IF:8.2

Published: 2023

Results: In the present study, Con A was extracted and purified from the seeds of C. ensiformis. The crude extract was obtained using a sodium chloride solution. The crude extract was obtained using sodium chloride solution, followed by affinity chromatography to purify Con A. Its purity was determined by gel electrophoresis (SDS-PAGE). The results showed that ConA displayed three bands: α chain (25.5 kDa), β chain (14 kDa), and γ chain (12 kDa).

Fig.2 ConA purification by affinity chromatography. (dos Santos, et al., 2023)Fig.2 ConA purification by affinity chromatography. (dos Santos, et al., 2023)

Applications of C. ensiformis Lectin Production

  • C. ensiformis lectin is found to have the potential ability for bacterial inhibition. C. ensiformis lectin produced by our optimized method is used in the research of fungicides and so on.
  • C. ensiformis lectin produced by our optimized method has potential application in the identification of tumors of different tissue origins.
  • C. ensiformis lectin has been used to identify, classify, and purify various glycoconjugates. C. ensiformis lectin produced by our optimized method is used to develop novel detection techniques.

Highlights of Us

  • The traditional extraction process for C. ensiformis lectin is cumbersome. Through optimization, we reduce the number of extraction steps, shorten the extraction time, and increase the yield.
  • The traditional lectin purification process is cumbersome and time-consuming when applied to microanalysis. We use the optimized purification method to improve the purification efficiency and product purity.
  • We have a wide range of C. ensiformis lectin to meet the needs of our clients for different applications.

CD BioGlyco aims to provide high-quality solutions for lectin production. With our rich experience in lectin production, we will provide the optimal production solution with short cycle time and high efficiency. If you need C. ensiformis lectin production, please feel free to contact us. We will fulfill all the experiments you request with excellence.

Reference

  1. dos Santos, A.M.D.; et al. Canavalia ensiformis lectin induced oxidative stress mediate both toxicity and genotoxicity in Drosophila melanogaster. Int J Biol Macromol. 2022, 222(Pt B): 2823-2832.
This service is for Research Use Only, not intended for any clinical use.

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