Next-generation Sequencing (NGS)-based Glycogene Expression Profiling

Introduction of Next-generation Sequencing (NGS)

The rapid update of RNA-seq technology has accelerated transcriptome analyses in new times with higher efficiency and lower cost. Unlike the first-generation sequencing methods based on Sanger sequencing, which used fluorescently labeled or radioactive dNTPs, the researchers used a recently discovered luminescent method named pyrosequencing for DNA sequencing. It laid the foundation for the emergence of NGS. NGS uses "sequencing by synthesis" technology, making it possible to sequence hundreds of thousands of nucleic acid molecules at once and analyze the transcriptome and genome of a species in detail. Therefore, it is also called high-throughput sequencing, which can easily detect unknown gene expression sequences, with many advantages such as fast speed, low cost, and high precision.

Fig.1 Milestone events in the field of RNA sequencing. (Hong, et al., 2020)

NGS-based Glycogene Expression Profiling at CD BioGlyco

CD BioGlyco has invested a lot of effort in RNA sequencing research and has a wealth of experience. The simple NGS workflow we provide is shown as follows:

Fig.2 Workflow of simple NGS. (CD BioGlyco)

CD BioGlyco offers different NGS-based glycogene expression profiling services. There are many opportunities to use NGS in your research:

• Compared to first-generation sequencing, NGS captures a wider range of mutations
• NGS analyzes the entire genome without bias
• NGS is used to detect rare cell variants, even if they are present in only a few percent of cells

Applications

• Rapid genome sequencing
• Identification of new RNA variants and splicing sites
• Quantifying mRNA for glycogene expression profiling
• Analysis of epigenetics such as genome-wide DNA methylation and DNA-protein interactions
• Study on rare cell variation and cell subcloning
• Study on the tumor microenvironment
• Detection biomarkers for diseases

Highlights

• Cutting-edge sequencing technology
• High-throughput and automated NGS process
• Accurate genomic analysis
• Visualized detection process
• Scientific and reliable data

It is mission-critical for CD BioGlyco to provide flexible, innovative, and scalable solutions to clients. As an international company that places a high value on collaborative interaction, fast delivery of solutions, and high-quality service, we are constantly improving. We have a comprehensive Glygenomics Platform to solve the challenges in glycobiology for our clients. If you are interested in our services, please feel free to contact us for more comprehensive information.