Development of Whole-genome Sequencing (WGS)

WGS was invented and applied to sequencing small genomes (4000-7000 bp) as early as 1979. But early WGS technology was slow, labor-intensive, and expensive. The emergence and widespread application of next-generation sequencing has greatly improved the availability of WGS due to its advantages of automation, high efficiency, low cost, and high throughput. The exponential growth of next-generation sequencing (NGS) data has driven a boom in the development and release of genomic analysis tools. WGS not only provides a better understanding of all functional genes in the cell but also provides more comprehensive genetic information by sequencing non-coding DNA, which makes up 98-99% of the genome. WGS shows powerful advantages in identifying single nucleotide variants (SNV), insertions and deletions (InDel), structure variants (SV), and copy number variants (CNV).

Fig.1 A workflow for rapid WGS. (Halabi, et al., 2022)

WGS-based Glycogene Expression Profiling at CD BioGlyco

Our WGS process provides fully functional and quick solutions for laboratory research and brings high-quality insights into your study of the whole genome.

Fig.2 Our WGS process and direction of its application. (CD BioGlyco)

Different methodologies to get genome sequence:

• De novo sequencing: The genome in a species is sequenced directly, and the obtained sequences are sorted and spliced to map the entire genome sequence of the species.
• Re-sequencing: The whole genome in one or some individuals of a species with known genomic information is sequenced, the sequence differences between individuals and populations are contrastively analyzed, and then single nucleotide polymorphism associated with phenotype can be identified.

With the support of the WGS technology, you more comprehensively and deeply clarify the important position of glycogenome in the whole genome, as well as the differential expression of glycogenes in diseases. It is conducive to your study on the glycogene function, the elucidation of the pathogenesis and malignant progression of diseases, especially tumors, and the development of new therapies.

Applications

• People: rare disease research; genetic disease analysis; Cancer Glycogene Discovery; pathogenic mutated gene discovery; pathogen resistance mechanism research.
• Microorganism: microbial analysis; study on mechanisms of population evolution; study on transmission chains of infectious diseases; epidemics prediction.
• Animal and plant: study on the origin and evolution of species; molecular breeding; study on genetic diversity of varieties; study on safety and stability.

Highlights

• Automated and high-throughput whole genome sequencing service
• The scientific and reliable analysis process
• Professional technicians
• End-to-end service

CD BioGlyco is a leader in the field of glycobiology. We have built an advanced Glycogenomics Platform and provide the most cutting-edge technology to support your needs in Glycogene Editing, Glycogene Delivery, and Glycogene Expression. Please feel free to contact us and we will be in touch with you as soon as possible.