The Roles of Oligonucleotide TET Labeling

In recent years, the production of oligonucleotides has increased dramatically due to the use of modified oligos in many biological applications, such as DNA sequencing, genotyping, and quantitative polymerase chain reaction (PCR). Dye fluorescent-labeled oligonucleotides include direct or indirect labeling, which is currently widely used in biotin labeling. TET is a Fluorescein derivative. TET and HEX are improved based on FAM. The chlorine atoms produce a certain redshift in both the absorption wavelength and the emission wavelength values of FAM and weaken the pH sensitivity to some extent. TET is also used in the Argon-ion Laser excitation light source, Abs/Em=521/536 nm. The choice of labels was further diversified according to the spectral requirements. This technique provides a novel method for simple, fast, and convenient point-of-care diagnostics for monitoring proteins and metal ions.

Fig.1 Schematic diagram of TET labeling test. (Cai, et al., 2019)

Oligonucleotide TET Labeling Services at CD BioGlyco

With the development of new methods for single nucleotide polymorphism detection and sensitive diagnostics based on vial assays for fluorescently labeled oligonucleotides, there is an urgent need for high-performance purification and modification techniques of these special oligonucleotide probes. CD BioGlyco offers nucleic acid probes labeled with TET services.

• Oligonucleotide TET labeling service

The services are classified into enzymatic labeling, chemical labeling, and direct labeling based on the different research needs of our customers, which rapidly and stably label TET dyes with oligonucleotides. The TET dye is quickly and stably labeled.

• Oligonucleotide TET-labeled product purification service

• Since the performance of genotyping and quantitative PCR assays is sensitive to the quality of fluorescent probes, it is crucial to purify these oligonucleotides before use. Our company generally adopts two methods of polyacrylamide gel electrophoresis (PAGE) and reversed-phase high-performance liquid chromatography (RP-HPLC) for purification, which effectively desalinates and removes impurities.
• The high-performance liquid chromatography is coupled with mass spectrometry, which is used for the characterization of oligonucleotide impurities to identify dye-labeled failure sequences. Their retention behavior was investigated, which is an effective tool for the semi-preparative purification of dye-labeled diagnostic oligonucleotides.

Fig.2 Schematic diagram of the main points of the TET labeling service. (CD BioGlyco)

Applications of TET Labeling

• TET-labeled oligonucleotide can be used for metal ions and pathogenic pathogens detection.
• TET-labeled oligonucleotide can be used for sequencing by hybridization.
• TET-labeled oligonucleotide can be used in the diagnosis of hereditary diseases and tumors.

CD BioGlyco is a biotechnology company that specializes in providing various Oligonucleotide Modifications and is well-regarded in the industry. According to the client's different synthesis purposes or methods of use, we help clients rationally plan labeling methods and purification methods. If you are interested in our service, please feel free to contact us further to introduce the specific service in detail.