PAGE-based Oligonucleotide Fragment Purification Service

Purification is a critical step in oligonucleotide synthesis is often time-consuming and requires complex procedures. The main factors that should be considered in the selection of purification methods are application direction, purity, sequence length, special modification, cycle time, cost, yield, etc. CD BioGlyco customizes targeted purification schemes according to specific oligonucleotide application directions. We will use mature purification technology to provide high-purity and reliable oligonucleotide products to better promote clients' studies.

Polyacrylamide gel electrophoresis (PAGE)-based Oligonucleotide Fragment Purification Services at CD BioGlyco

PAGE means that under a certain electric field strength, samples will be separated according to different lengths after electrophoresis through denaturing polyacrylamide gel. Therefore, after the sample passes through electrophoresis, the band of the target sequence is cut off, and then the sequence is released from the gel by a suitable solvent, to obtain the target sequence with higher purity. This method can be used for sequence purification of different lengths and different application scenarios.

CD BioGlyco provides the following PAGE-based oligonucleotide sequence fragment purification services.

• Sample Analysis
  CD BioGlyco analyzes the oligonucleotide by PAGE technology to determine its size, purity, and existing impurities, which can help assess sample quality and suitability, and provide a baseline for subsequent purification steps.
• Separation of Fragments
  CD BioGlyco offers to purify and separate target oligonucleotide fragments of specific sizes or sequences by using PAGE technology.
• Pretreatment
  CD BioGlyco provides oligonucleotide pretreatment and pre-purification services to improve purification, such as removing salts, solvents, and other impurities, and concentrating samples.
• PAGE Purification
  Oligonucleotides are loaded into a polyacrylamide gel. We apply an electric field for electrophoretic separation and then cut the purified target fragment from the gel.
• Post-purification Treatment
  Purified oligonucleotide fragments may require some post-processing steps to obtain the final pure product, such as elution, concentration, removal of gel residue, etc.
• Analysis and Verification
  Purified oligonucleotide fragments often require further analysis and verification to ensure their quality and purity, which can include the use of various techniques such as electrophoretic analysis, mass spectrometry, colorimetry, or spectroscopic analysis.

Fig.1 Multiple types of oligonucleotide fragment purification. (CD BioGlyco)

In addition, CD BioGlyco also provides other purification services, including Desalination-based Oligonucleotide Fragment Purification, Cartridge (OPC)-based Oligonucleotide Fragment Purification, and HPLC-based Oligonucleotide Fragment Purification.

Applications

• Oligonucleotide modification research: By electrophoresis separation, highly pure and specifically modified oligonucleotide fragments can be obtained for studying the impact of modification on structure and function.
• Oligonucleotide synthesis: Separate sequence fragments of different lengths or sequences by electrophoresis to remove impurities and by-products during the synthesis process.
• DNA sequencing: Separation by electrophoresis can be used to purify PCR amplification products and improve the accuracy and reliability of sequencing results.
• RNA research: Through electrophoresis separation, RNA fragments of specific size and purity can be obtained for functional research, structural analysis, and drug development.

Advantages

• CD BioGlyco performs high-efficiency separation according to the size and charge characteristics of sequence fragments, and provides high-purity target fragments.
• According to the size, sequence, and modification of the target fragment, a specific purification scheme can be formulated to meet the needs of clients.
• PAGE purification technology provided by our professional laboratories ensures consistent and high-quality purification results.

CD BioGlyco provides reliable oligonucleotide fragment purification services. Please feel free to contact us in time if you have any needs for our oligonucleotide purification services.