Synthetic oligonucleotides are in increasing demand in fields ranging from clinical diagnostics to novel biopharmaceutical therapeutics. The synthesis of oligonucleotides is an efficient process, and small amounts of impurities are produced during each step of the synthesis. Therefore, manufacturing organizations as well as individuals who rely on the quality of oligonucleotides have shown interest in developing effective and efficient methods for the purification and analysis of these important biological aptamers. For researchers, there is also a need to use oligonucleotides of higher purity. Failure to purify crude synthetic oligonucleotides can seriously hamper an organization's or individual's ability to achieve desired results. Therefore, oligonucleotides used for gene knockout, genotyping, and diagnostic purposes often require further purification after synthesis.
Fig.1 Typical procedure for the extraction of oligonucleotides. (Nuckowski, et al., 2018)
CD BioGlyco has a professional Glyco™ Synthesis Platform with multiple world-leading technologies to provide clients with outstanding Custom Carbohydrate Synthesis. CD Bioglyco provides technologies based on C-18 reversed-phase high-performance liquid chromatography, technologies based on normal-phase chromatography, and technologies based on gel filtration to desalt oligonucleotides. Desalting is a very basic oligosaccharide purification step. In this step, excess salt is removed from the mixture by using normal-phase chromatography. It will not eliminate the fault sequence. It produces salt-free, ready-to-use DNA solutions ideal for robust techniques such as polymerase chain reaction (PCR), amplified restriction fragment polymorphism analysis, microarrays, and more. Desalting also allows for use in combination with other purification techniques.
Additionally, we offer chemical-based methods for desalting oligonucleotides. For example, oligonucleotides are desalted by ethanol precipitation. Briefly, an equal volume of water is added to a solution of triethylamine trihydrofluoride, which is then diluted with slightly acidic sodium acetate. After adding 100% ethanol for precipitation, followed by high-speed centrifugation.
Fig.2 Desalination-based oligonucleotide fragment purification technology. (CD BioGlyco)
CD BioGlyco is committed to providing the best solutions for Custom Oligonucleotide Synthesis for global clients. We provide high-quality Oligonucleotide Fragment Purification Services for clients all over the world. If you are interested in our services, please feel free to contact us for more details.
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