Custom Oligonucleotide Synthesis Service

Applications of Oligonucleotides in Different Fields

Oligonucleotides are usually composed of up to 20 short-chain nucleotides (deoxyribonucleotides or ribonucleotides). They have a variety of primer lengths. It can be used in pre-designed, customized, or randomized forms for applications in microarrays, gel migration analysis, artificial gene synthesis forensic medicine, etc. There are many types of current research, such as tiny ribonucleic acids, small interfering ribonucleic acids, nucleic acid aptamers, etc. They can achieve very high selectivity by pairing with DNA, mRNA, or pre-mRNA. Based on this, oligonucleotide has a variety of uses. It can be used in diagnostic tests, antisense agents in target validation, competitive inhibitors of transcription factors, and so on. It can also be used as a stimulator of the immune system or as a biotherapeutic agent for the treatment of diseases. In addition, it can precisely inhibit certain genes, silencing genes that code for abnormalities, thus preventing the expression of many faulty proteins.

Custom Oligonucleotide Synthesis Service at CD BioGlyco

CD BioGlyco has excellent synthetic capabilities to meet a variety of custom synthesis needs. Synthetic oligonucleotides avoid the disadvantages of the natural. Due to some shortcomings of the traditional solid-phase methods, such as limited length and purity, scientists began to look for new synthesis methods. At present, we mainly use two methods of chemical synthesis and biological enzyme synthesis to produce.

Fig.1 Oligonucleotide R&D services. (CD BioGlyco)

• Oligonucleotide Synthesis Services
  Oligonucleotides are usually prepared by: stepwise addition of nucleotide residues to produce a particular sequence. We synthesize various products using both chemical and biological methods. With advanced synthesis technology and laboratory equipment, we are capable of providing products of various specifications. We produce not only unmodified, standard products but also complex and various modified products based on our Oligonucleotide Modification Service.

Fig.2 Oligonucleotide synthesis process. (CD BioGlyco)

• Oligonucleotide Purification Service
  There are some side reactions during synthesis. The target products need to be isolated. Purification and characterization are currently a major hot spot in the pharmaceutical industry. Currently, the commonly used purification methods include four kinds: OPC column purification, HPLC purification, direct desalting purification, PAGE purification, etc. We have optimized various purification steps. According to client requirements, we will provide the corresponding purification method service.

Applications of Custom Oligonucleotide Synthesis

• Plant chromosomes can be studied using the developed oligonucleotide probes. The study of plant chromosomes can ultimately provide a cytological basis for elucidating plant classification, origin, evolution, phylogeny, genetics, and so on.
• The occurrence of cancer is closely related to the abnormal regulation of signaling pathways, etc. Antisense oligonucleotide molecules are used to study the effects of cancer growth, metastasis, etc., in vitro and in vivo.
• Oligonucleotide primers can be used for gene cloning, sequencing, etc. Through its inhibition or silencing of gene expression enables screening for genetic diseases or identification of specific pathogens.

Advantages of Us

• We have excellent chemical and enzymatic synthesis capabilities. We monitor chemical reactions in real time to ensure optimal product quality.
• Enzymatic methods offer several advantages. Enzymatic reactions allow small fragments to be joined to become large fragments, thus speeding up the synthesis process.
• Oligonucleotides have a wide variety of biological applications, etc. Both natural and modified oligonucleotide products can be synthesized.

CD BioGlyco aims to facilitate drug research, molecular probe assay development, and so on. We also provide production services such as Polynucleotide and Nucleoside-based Production. To learn more about our products and how to synthesize them, please feel free to contact us.