Nucleoside-based Production Service
A nucleoside is an organic molecule that is made up of a nitrogenous base and a sugar molecule. The sugar within a nucleoside can be either ribose or deoxyribose, which is attached by glycoside bonds to the nitrogen-containing base. It can be thought of as a nucleotide that has no phosphate group. Nucleosides are important parts of DNA and RNA and play a crucial role in the storage and transmission of genetic information. Nitrogenous bases in nucleosides can be one of a few types, including adenine (A), guanine (G), cytosine (C), thymine (T), and uracil (U). A particular composition of nitrogen-containing bases and sugar molecules determines the type of nucleoside. Nucleosides play an essential role in cellular functions. They are engaged in DNA copying, transcription, and translation, which are vital for the storing and expression of genetic information. Nucleosides also have other functions in cell signaling and metabolism.
We employ advanced chemical synthesis and purification techniques to produce diverse sugar-nucleosides. Its core innovation is a novel method utilizing 2'-deoxyribonucleoside 5'-phosphites as monomer units, fundamentally differing from traditional phosphoramidite/H-phosphonate approaches.
A key advancement is the use of base-unprotected 2'-deoxyribonucleoside 5'-phosphites. This eliminates complex nucleobase protection/deprotection steps, streamlining synthesis while boosting efficiency and reducing side reactions. These monomers are synthesized in a simple, one-step reaction from parent nucleosides.
The synthetic cycle involves robust chain elongation: the monomer's 3'-OH group condenses with the growing oligomer's 5'-terminal H-phosphonate monoester. Critical to this cycle is the rapid, quantitative removal of phosphite protecting groups to regenerate the H-phosphonate monoester for subsequent condensations.
This methodology enables the synthesis of diverse sugar-nucleosides and analogs, providing a versatile platform for clients.
There are many different types of nucleosides, and CD BioGlyco offers nucleosides production services that include, but are not limited to:
Nitrogenous bases are the essential components of nucleosides. At CD BioGlyco, we offer nucleoside production services on adenine, cytosine, guanine, uracil, and thymine. We have both biological and chemical routes to choose from in the production of nitrogenous bases, and we offer custom production solutions to each of our clients.
Ribonucleosides play an important role in living organisms and are part of RNA. CD BioGlyco offers ribonucleoside-based production of adenosine, cytosine, guanosine, uridine, and 5-methyluridine. We have multiple routes for each riboside and are constantly optimizing our production solutions to ensure the most efficient production for our clients.
Another part of nucleosides is deoxyribonucleoside, which is also a component of DNA. At CD BioGlyco, we offer services in the production of deoxyadenosine, deoxyguanosine, thymidine, uridine, and deoxycytidine. During the production process, we provide our clients with the most suitable production solution.
Nucleoside-based derivatives of nucleosides are also important for the human body. For this reason, we also offer nucleoside-based derivative production services.
Our sugar-nucleoside production service is designed as a collaborative and efficient process to ensure the highest quality results for your project.
The process starts with a detailed consultation with our expert scientific team. We work with you to define your specific needs, including the exact structure of the sugar-nucleoside, desired quantity, and purity requirements. We then design a custom synthesis plan to meet these specifications.
We begin by preparing the necessary monomer units, employing a simple one-step reaction from base-unprotected 2'-deoxyribonucleosides. For nucleosides that are less soluble, like 2'-deoxycytidine and 2'-deoxyguanosine, we use co-solvents such as N-methylpyrrolidone (NMP) with pyridine to significantly improve solubility and enhance reaction selectivity.
This stage involves the sequential addition of monomer units to build the target sugar-nucleoside or oligonucleotide. Our chain elongation cycle includes three key steps: deprotection, condensation, and capping. The use of H-phosphonate intermediates enables the synthesis of a wide variety of DNA analogs that are not accessible via other methods.
After synthesis, the crude product undergoes a rigorous purification process. We leverage advanced chromatographic techniques, and in some cases, the monomer units can be easily purified by crystallization. Each batch is then subjected to a comprehensive quality control assessment, including NMR, HPLC, and mass spectrometry, to confirm its identity, structure, and purity.
The high-purity, quality-controlled sugar-nucleoside is delivered with a detailed certificate of analysis (CoA), providing you with complete confidence in the product's quality and specifications.
DOI.: 10.3390/molecules27113499
Journal: Molecules
IF: 4.6
Published: 2020
Results: This study synthesized seven modified sugar and nucleoside analogs (compounds 2-8) from α-D-glucose through a 21-step pathway. The compounds were evaluated for analgesic activity in mice using acetic acid-induced writhing assays and for cytotoxicity against HeLa cancer cells via trypan blue exclusion and CCK-8 assays. Key findings include:
Our nucleosides are used in the synthesis of antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), and aptamers for targeted gene regulation and therapeutic intervention.
Many antiviral and anticancer drugs are nucleoside analogs, and our service is critical for the research of these therapeutic agents.
Modified nucleosides are vital tools for studying DNA and RNA structure, function, and epigenetic modifications, offering insights into gene expression and disease mechanisms.
Our nucleoside production services provide high-purity molecular building blocks (adenine, cytosine, guanine, uracil, thymine) essential for synthesizing oligonucleotide therapeutics. To transform these nucleosides into functionally advanced constructs, we offer precision Oligonucleotide Modification Services that enhance stability, targeting, and therapeutic efficacy:
CD BioGlyco is a company with extensive experience in the manufacturing of nucleosides and their derivatives. Our versatile analytical platform and specialized researchers provide custom production services. If you are interested in our services, please do not hesitate to contact us.
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