Polynucleotide Synthesis Service

Polynucleotide Is an Important Substance in Nature

Polynucleotide is a natural polymer that contains 13 or more nucleotide monomers. They are linked by covalent bonds. They form deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

Fig.1 The schematic diagram of polynucleotides. (Noreen, et al., 2020)

Our Polynucleotide Synthesis Service at CD BioGlyco

CD BioGlyco offers a comprehensive range of techniques for the custom synthesis of DNA and RNA and provides diverse options including chemical custom synthesis and enzymatic custom synthesis.

Custom DNA Synthesis Service

• Our chemical approach for DNA synthesis couples DNA phosphoramidite nucleosides to a solid support and utilizes a four-step cycle of deblocking, activation, capping, and oxidation to construct the oligonucleotide chain.
• In our enzymatic synthesis, we use terminal deoxynucleotidyl transferase (TdT) as the primary enzyme for DNA synthesis.
• In addition to our DNA synthesis services, we offer the synthesis of DNA hybrids incorporating various inorganic nanomaterials such as gold, magnetic nanoparticles, and silver.

Custom RNA Synthesis Service

• We offer a solid-phase chemical synthesis approach by using an automated synthesizer. In our method, the RNA chain is elongated step by step on a solid support. We efficiently and precisely synthesize RNA molecules with high purity and yield.
• Our in vitro transcription method uses a DNA template with a T7 RNA polymerase promoter. As a result, our method facilitates efficient synthesis of RNA molecules, leading to high yields usually in the milligram range.

DNA and RNA Purification Service

In addition, we provide DNA and RNA purification services to guarantee purified DNA and RNA for downstream research and applications. We effectively eliminate any unwanted by-products and impurities that may occur during the productive process by our technologies, including PAGE-based Purification Service and HPLC-based Purification Service.

Fig.2 The technologies of purification. (CD BioGlyco)

Applications

• Interference RNA is applied for researching the functions of genes in cell culture and model organisms
• Recombinant DNA can be used to produce human insulin, eliminating the reliance on animal sources and enabling its widespread usage in the pharmaceutical industry.
• DNA technology is useful in agriculture. It enhances crop yield by transferring certain genes and increases nutrition value and shelf life by modifying certain genes.
• DNA can be used in RFLP and STR to identify suspects.

Advantages

• We utilize advanced polynucleotide synthesis techniques and optimal synthesis methods to ensure the accuracy and integrity of the structures.
• Our synthesis process undergoes rigorous quality control measures to provide reliable and consistent results.
• We have advanced PAGE and HPLC technologies to purity those polynucleotides we custom to ensure our quality of service.

CD BioGlyco is a leader in the field of glycobiology, we are committed to offering carbohydrate synthesis services to meet your specific needs. We provide accurate and efficient synthesis solutions with our advanced techniques. With our simple processes and efficient project management, we guarantee the perfect completion of the service within the agreed time. Feel free to contact us as soon as possible if you are interested in our services.