Pleurotus ostreatus lectin (POL) is derived from the fruiting body of edible oyster mushrooms and belongs to Fungal Lectin. It is a dimer of molecular weight 80 kDa consisting of two polypeptide chains and disulfide bonds. Each domain of POL contains carbohydrate binding sites since calcium. Notably, POL specifically recognizes galactose residues and is tightly associated with galactosidase activity. The binding effect of POL and carbohydrates is closely related to its 3D structure. Therefore, CD BioGlyco provides a series of services, such as POL extraction, separation, and identification, to accelerate the client's project progress in lectin research.

POL Extraction and Purification Service at CD BioGlyco

All the oyster mushrooms are harvested at the mushroom hothouse to ensure freshness. Each step is performed at 4°C. The fruiting bodies are homogenized with water and extracted with stirring. The suspension is centrifuged and the supernatant, as well as sediment, are gathered in isolation. The supernatant lyophilized powder is dissolved in a buffer. Then purified using an agarose gel column. During the washout process, collect tube by tube. All fractions are freeze-dried and reconstituted, followed by the addition of solid ammonium sulfate and centrifugation to collect the precipitate. Redissolve in distilled water and dialyze. The target components are collected after a secondary purification using an affinity chromatography column. The fractions are dialyzed with distilled water and lyophilized to obtain high-purity POL.

POL Analysis Service at CD BioGlyco

The development of its functionality and application characteristics requires a thorough analysis of the POL. For purified POL, we provide precise analysis services to clients. Our professional team has a wealth of proven experience, so feel free to leave your samples in our hands!

Fig.1 Schematic diagram of POL analysis services. (CD BioGlyco)

Publication

Applications of POL

• POL shows obvious anti-liver cancer and anti-tumor activity in mice, and it is suitable for anticancer and anti-tumor drug development.
• POL exhibits antiproliferative effects in human cancer cell lines in vitro and is a potential cancer therapeutic tool.
• POL is a naturally active protein. It stimulates innate responses by activating the Toll-like receptor signaling pathway in dendritic cells, overcoming the HBV tolerance. POL has potent immune activity as a novel target for HBV tolerance therapy.
• Immobilize the POL (magnetic bead, Microarray Assay), binding to the bovine submaxillary mucin (BSM) to analyze the binding specificity of various monosaccharides or oligosaccharides to lectins.

CD BioGlyco provides Lectin Production Services and reliable data supported by a team of specialists. We emphasize the quality of natural products, and all extraction and separation services are carried out under strict conditions. Please feel free to contact us.