CD BioGlyco has developed a variety of fluorimetric methods to provide our clients with sialic acid analysis services. We are confident to be your scientific assistant in the field of glycobiology.
Sialic acid plays an important role in pathology and physiology, so it is important to develop accurate, simple, and rapid methods for sialic acid quantification and analysis. Currently, many methods have been reported to determine total sialic acids in biological materials, such as fluorometric assay and fluorescence imaging.
Fluorometric assay, which has been used to analyze sialic acid and its derivatives, typically involves the reaction of sialic acid with an oxidant or acid, and the resulting compounds further react to form a fluorophore. It is then excited, and the emission is measured to determine the sialic acid content in the sample. Fluorescence imaging is the visualization of fluorescent dyes or proteins as markers for molecular processes or structures. Particularly, fluorescence imaging proposes a convenient visualization method for the analysis of sialic acids.
CD BioGlyco has developed various fluorimetric methods for the detection and quantitative analysis of sialic acids, including fluorometric assays and fluorescence imaging. We measure the content of sialic acids using fluorometric assays including 3,5-diaminobenzoic acid, thiobarbiturate, pyridoxamine, acetylacetone/periodic acid, and acetoacetanilide/ammonia. The assay of 3,5-diaminobenzoic acid is highly specific for sialic acid and therefore provides accurate results in the quantification of sialic acids in human samples. The assay of thiobarbiturate is 500-fold more sensitive than the traditional thiobarbiturate method, allowing for analysis of sialic acid in the picomole range. The assay of pyridoxamine is specific for free sialic acid, but bound sialic acid can also be measured by performing a previous acid release step. The assay of acetylacetone could be used to quantify O-acetylated sialic acid. The assay of acetoacetanilide is extremely sensitive and can measure quantities of sialic acid as low as 1 μg and as high as 90 μg.
We have synthesized fluorescently labeled molecularly imprinted polymers for imaging of human keratinocytes to localize and quantify hyaluronan and sialylation moieties on and in the cells. According to the characteristics of intracellular or in vivo sialylated glycans, we have designed and synthesized a novel sialic acid-imprinted conjugated polymer nanoprobe with two-photon fluorescent properties, which exhibits specific recognition ability to the target sialic acid and has been used for monitoring intracellular sialylated glycans levels selectively. We also provide the synthesis of polymer chain wound nanoclusters for fast targeting and mapping of sialic acids under the fluorescent microscope.
CD BioGlyco has many successful experiences in sialic acid fluorescence analysis. If you have sialic acid analysis projects by fluorescence, please contact us for more detailed information.
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