Sialic Acid-binding Lectin Production Service

Sialic Acid-binding Lectin Production Services at CD BioGlyco

Recombinant lectins have more targeted biological functions than natural lectins. CD BioGlyco has a long-term commitment to the Production of Various Types of Lectins, which includes the production of different types of Recombinant Lectins. For sialic acid-binding lectin, we offer production services that include but are not limited to the following.

• Production of sialic acid-binding lectin

We use specific bacteria as hosts to construct lectin expression vectors and genetically engineer them to produce sialic acid-binding lectin in their C-terminal structural domains using galactose-binding lectin as a precursor. In this process, Gly and Ser residues act as flexible linkers for constructing tandemly repetitive sequences of sialic acid-binding lectin plasmids.

• Purification of sialic acid-binding lectin

We purify sialic acid-binding lectin using Affinity Chromatography. After purification, the lectin is purified again by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and lactose-agarose column. Finally, high-purity sialic acid-binding lectin is obtained.

• Analysis of sialic acid-binding lectin
  • Coagulant activity analysis
    Use buffer to dissolve the lectin, add suspensions of different bacteria or animal erythrocytes to it, and observe the agglutination activity of the lectin on different bacteria and animals under the microscope.
  • Sugar binding assay
    Different sugar solutions are added to the lectin samples, and each mixture is analyzed for affinity between the lectin and the sugar, and the inhibitory activity of the sugar on the lectin, using a lectin immobilization column.
  • Glycoprotein binding assay
    We utilize glycoprotein imprinted microarrays to detect the binding signals between the lectin and different glycoproteins by a sudden field fluorescence scanner, which is used to test the binding ability of the lectin to different glycoproteins.

Fig.1 Our sialic acid-binding lectin production services. (CD BioGlyco)

In the production of sialic acid-binding lectin, we also offer the production of the following related lectins.

Applications

• Sialic acid-binding lectin is used in research on a variety of immune activities, such as regulation of inflammatory responses, leukocyte proliferation, host-microbe interactions, and cancer immunity.
• Sialic acid-binding lectin binds to glycoproteins, and it is used as a powerful probe for salivary glycomics to detect glycoproteins.
• Sialic acid-binding lectin is used in the development of coagulants.

Advantages

• We provide the most reliable custom revision strategy and flexible service model, so clients choose the most suitable solutions and services according to their needs.
• We are committed to completing the whole process of service with high efficiency and high quality, and do our best to meet our client's time requirements.
• Our products are of high purity and high stability to ensure that they meet the research needs of our clients.

CD BioGlyco has advanced lectin production technology and offers custom lectin production solutions based on client requirements. Our services will help you expand the functional diversity of your lectins and open up more possibilities for your research. Please feel free to contact us if you would like to learn more about our service.