High-quality Lectin Production Services at CD BioGlyco

Lectin has a wide variety of types and its applications are expanding. CD BioGlyco is dedicated to the Production of Lectins with advanced technology. We provide not only Natural Lectin Production Services but also various types of Recombinant Lectin Production Services. For example, in the production of α2-3-linked sialic acid-binding lectins, we will also provide two specificity production services: terminal α2-3-linked sialic acid (Neu5Ac)-on N-linked and O-linked glycans, and terminal α2-3-linked sialic acid (Neu5Ac)-on O-linked glycans.

• α2-3-linked sialic acid-binding lectins preparation

We synthesize α2-3-linked sialic acid-binding lectin by constructing expression vectors in specific cells using genetic engineering methods.

In this case, salivary acid residues are present at the outermost ends of the sphingolipids, and N- and O- glycans bind directly to the salivary acid residues to obtain glycans with terminal α2-3-linked salivary acid in N- and O-linked. On the other hand, in the above process, the doping-protection-deprotection method, which occupies the N-terminus of the glycan, prepares a glycan with terminal α2-3-linked sialic acid (Neu5Ac)-in O-linked.

• α2-3-linked sialic acid-binding lectins purification

We utilize a Gel Filtration column and an Affinity Chromatography column to purify the lectin. Pure lectin is obtained by collecting the product with a strong absorption peak in a split tube, as well as using a concentration and drying operation.

• α2-3-linked sialic acid-binding lectins analysis
  • Agglutination activity
    The hemagglutination method is used to test the agglutination activity of this lectin. The process is as follows: add the red blood cell suspension of the animal in the lectin solution, and after a while, observe the coagulation state in the mixture under the microscope or directly with the naked eye to get the agglutination activity of the lectin.
  • Molecular weight and structure
    The subunit composition, protein content, and molecular weight of the lectins are analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and ultraviolet spectroscopy.
  • Sugar binding properties
    The sugar-binding capacity of the lectin is analyzed using glycoconfiguration microarrays and affinity chromatography, and the binding capacity of different sugars are also compared.

Fig.1 α2-3-linked sialic acid-binding lectin production services. (CD BioGlyco)

Applications

• The α2-3-linked sialic acid-binding lectin has sugar-binding properties, which allow it to be used to detect and isolate specific sugar molecules.
• The α2-3-linked sialic acid-binding lectin is used as a probe for cell surface markers to research cell recognition and transformation processes.
• α2-3-linked sialic acid-binding lectin is used as a diagnostic and therapeutic target for certain diseases.

Advantages of Us

• We provide lectin products with high purity and reliable quality to ensure that we meet the scientific needs of our clients.
• Our service team is equipped with advanced production technology to provide custom lectin production solutions for our clients.
• Our production services include all aspects of lectin preparation, purification, and analysis, improving efficiency and saving time for our clients.

At CD BioGlyco, we have a research and development team focused on multiple types of lectins. For example, Sialic Acid-binding Lectin. Our team is committed to maintaining the highest standards, providing each client with a custom lectin production solution, and doing our best to help our clients solve their research challenges. Please feel free to contact us at any time, we will continually provide full support.